Owsley is a surname. Notable people with the surname include:
Alvin M. Owsley (1888–1967), American diplomat Augustus Owsley Stanley (1867–1958), Kentucky politician; campaigned against alcohol prohibition in the 1920s; grandfather of Owsley/Bear; descendant of William Owsley Augustus Owsley Stanley III (1935–2011), better known as Owsley Stanley, American audio engineer and clandestine chemist Bryan Owsley (1798–1849), Kentucky politician Charles Henry Owsley (1846–1935), English-born American architect Douglas W. Owsley (born 1951), forensic anthropologist and Division Head of Physical Anthropology at the Smithsonian Institution's National Museum of Natural History Frank Lawrence Owsley (1890–1956), American historian Henry Furlow Owsley III, American investment banker Jack Owsley (1883–1953), American football player, coach and businessman Lily Owsley (born 1994), English field hockey player Monroe Owsley (1900–1937), American actor Perry L. Owsley (1915–1979), American judge William Owsley (1782–1862), Kentucky politician, ancestor of Augustus Owsley Stanley and his grandson, Owsley Stanley/Bear William Owsley (Montana politician) (1842–1919), American politician