Gobionidae is a monophyletic family of benthic Eurasian cyprinoid fishes. This is a species rich clade which, as a subfamily of the Cyprinidae was divided into five tribes: Gobionini, Pseudogobionini, Hemibarbini, Coreiini, and Sarcocheilichthyini. These subdivisions are not recognised by Eschmeyer's Catalog of Fishes.

To adapt to different masticatory operations (food processing), members of the Gobioninae developed various types of pharyngeal bones and associated teeth; some have intermediate pharyngeal bones with rows of diverse teeth (conical, compressed, and coarsely compressed), others have broad pharyngeal bones with a single row of molariform teeth. Some Gobioninae have narrow pharyngeal bones with a row of extremely compressed teeth.

Genera These genera are included in the subfamily Gobioninae according to Eschmeyer's Catalog of Fishes:

Abbottina D. S. Jordan & Fowler, 1903 Acanthogobio Herzenstein, 1892 Belligobio D. S. Jordan & Hubbs, 1925 Biwia D. S. Jordan & Fowler, 1903 Coreius D. S. Jordan & Starks, 1905 Coreoleuciscus Mori, 1935 Gnathopogon Bleeker, 1860 Gobio Cuvier, 1816 Gobiobotia Kreyenberg, 1911 Gobiocypris M. R. Ye & T. Y. Fu, 1983 Hemibarbus Bleeker, 1860 Ladislavia Dybowski, 1869 Mesogobio Bănărescu & Nalbant, 1973 Microphysogobio Mori, 1934 Paracanthobrama Bleeker, 1864 Paraleucogobio Berg, 1907 Placogobio V. H. Nguyễn, 2002 Platysmacheilus Y. L. Lu, P. Q. Luo & Yi-Yu Chen, 1977 Pseudogobio Bleeker, 1860 Pseudopungtungia Mori, 1935 Pseudorasbora Bleeker, 1860 Pungtungia Herzenstein, 1892 Rhinogobio Bleeker, 1870 Romanogobio Bănărescu 1961 Sarcocheilichthys Bleeker, 1860 Saurogobio Bleeker, 1870 Squalidus Dybowski, 1872 Xenophysogobio Yi-Yu Chen & W. H. Cao, 1977

The extinct gobionines †Protothymallus Laube, 1901 and †Varhostichthys Obrhelová, 1969 are known from the Early to Late Oligocene of Europe.

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