Matherne is a French surname. Notable people with the surname include:
Beverly Matherne (born 1946), American writer and poet Samantha Matherne, American philosopher Wayne Matherne (born 1949), retired Canadian football player Brian Matherne, a coach in a sex abuse case in the archdiocese of Gregory Michael Aymond Ethel Jeanne Matherne (1916–2002), wife of educator and businessman Cliff Ammons Londa Jean Matherne, wife of Harry Connick Sr.