Aylesworth is an English surname. Notable people with this surname include the following:
Allen Bristol Aylesworth (1854–1952), Canadian lawyer and parliamentarian Arthur Aylesworth (1883–1946), American actor John Aylesworth (1928–2010), Canadian television producer and comedian Michael Aylesworth (b. 1943), American politician Reiko Aylesworth (b. 1972), American film and television actress Wilbert Ross Aylesworth (1892–1973), Canadian politician, farmer and merchant