tRNA pseudouridine32 synthase (, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine32 uracil mutase. This enzyme catalyses the following chemical reaction
tRNA uridine32 tRNA pseudouridine32
The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA.
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