Knowlton is a surname. Notable people with the surname include:
Andrew Knowlton, American magazine editor and television personality Austin Eldon Knowlton (1909–2003), American architect Bill Knowlton (1898–1944), American baseball pitcher Charles Knowlton (1800–1850), American physician and writer Daniel Gibson Knowlton (1922–2015), American bookbinder David Clark Knowlton, American social anthropologist and contributor to Mormonism: A Historical Encyclopedia Ebenezer Knowlton (1815–1874), American politician and minister Edward U. Knowlton (1933–2016), American physician and politician Elizabeth Knowlton (1895–1989), American mountaineer and writer Frank Hall Knowlton (1860–1926), American paleobotanist Grace Knowlton (1932–2020), American sculptor James H. Knowlton (1813–1879), American politician Ken Knowlton, American computer graphics pioneer and artist Miles Justice Knowlton (1825–1874), American missionary Nancy Knowlton (1949–), American biologist Richard Knowlton, former CEO of the Hormel company Thomas Knowlton (1740–1776), American military officer William A. Knowlton (1920–2008), American army general Wiram Knowlton (1816–1863), American politician