Bertolino is an Italian surname. Notable people with the surname include:
Cláudio Bertolino (born 1963), Brazilian racewalker Enrique Bertolino (1912–1997), Argentine golfer James Bertolino (born 1942), American poet Jean Bertolino (born 1936), French journalist and writer John Bertolino (1914–2003), American photojournalist Mónica Bertolino (born 1957), Argentinean architect Damián González Bertolino (born 1980), Uruguayan writer
See also Bertolin Bertolini Barbera bianca