2-nitroimidazole nitrohydrolase (, NnhA, 2NI nitrohydrolase, 2NI denitrase) is an enzyme with systematic name 2-nitroimidazole nitrohydrolase. This enzyme catalyses the following chemical reaction

2-nitroimidazole + H2O imidazol-2-one + nitrite

This enzyme is present in the soil bacterium Mycobacterium sp. JS330

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