Holford is a surname. Notable people with the surname include:
Castello Holford (1845 - 1905), American author of Aristopia David Holford (born 1940), West Indian cricketer George Holford (1860–1926), British soldier and courtier Karen Holford, Welsh engineer and academic Ingrid Holford (1920–2012), English meteorologist and author John Holford (1909–1997), Royal Navy medical officer Margaret Holford (the elder) (1757–1834), English novelist, playwright, and poet Margaret Holford (1778–1852), (also published as Margaret Hodson), English poet and translator Michael Holford (born 1983), English rugby player Patrick Holford, controversial British nutritionist Robert Stayner Holford (1808–1892), British politician Thomas Holford (1541–1588), Catholic priest and martyr Tom Holford (1878–1964), English footballer William Holford, Baron Holford (1907–1975), British town planner