3α(S)-strictosidine β-glucosidase () is an enzyme with systematic name strictosidine β-D-glucohydrolase. It catalyses the following chemical reaction:
The enzyme characterised from Catharanthus species removes the glycoside unit from strictosidine to give strictosidine aglycone and D-glucose. Strictosidine aglycone is a precursor to thousands of other indole alkaloids and this enzyme has been called the "gatekeeper" to this diversity.
The enzyme is a hydrolase, specifically a glycosidase that hydrolyses O- and S-glycosyl compounds.
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