endo-1,3-β-Xylanase, [xylanase (ambiguous), endo-1,3-β-xylosidase, 1,3-β-xylanase, 1,3-xylanase, β-1,3-xylanase, endo-β-1,3-xylanase, 1,3-β-D-xylan xylanohydrolase, xylan endo-1,3-β-xylosidase (misleading)] is an enzyme with systematic name 3-β-D-xylan xylanohydrolase. It catalyses the following chemical reaction:

Random endohydrolysis of (1→3)-β-D-glycosidic linkages in (1→3)-β-D-xylans

The enzyme is found mostly in marine bacteria, which break down the β(1,3)-xylan found in the cell wall of some green and red algae. It produces mainly xylobiose, xylotriose and xylotetraose.

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