The enzyme 4-sulfomuconolactone hydrolase (EC 3.1.1.92; systematic name 4-sulfomuconolactone sulfohydrolase) catalyses the chemical reaction:
The enzyme characterised from Hydrogenophaga intermedia and Agrobacterium radiobacter S2 hydrolyses the 4-sulfomuconolactone to maleylacetic acid and sulfurous acid. The reaction is part of the degradation pathway for 3,4-dihydroxybenzenesulfonic acid in these bacteria. The bacterial co-culture is able to metabolise other compounds such as 4-aminobenzenesulfonic acid.
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