Middlebrook is a surname. Notable people with the surname include:
Diane Middlebrook (1939–2007), American biographer, poet and teacher James Middlebrook (born 1977), English cricketer Jason Middlebrook (baseball) (born 1975), American baseball pitcher Lindsay Middlebrook (born 1955), Canadian ice hockey goaltender Martin Middlebrook (1932–2024), British military historian and author R. D. Middlebrook (1929–2010), early pioneer of power electronics William Middlebrook, 1st Baronet (1851–1936), English solicitor and Liberal Party politician Willie Middlebrook (1858–1919), English cricketer Willie Middlebrook (artist) (1957–2012), African-American photographer, artist and advocate
See also Middlebrooks, surname Middlebrook (disambiguation)
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