2-deoxystreptamine glucosyltransferase (, kanF (gene)) is an enzyme with systematic name UDP-alpha-D-glucose:2-deoxystreptamine 6-alpha-D-glucosyltransferase. It catalyses the following chemical reaction

This is an early step in the biosynthesis of the aminoglycoside antibiotics, kanamycin B and kanamycin C in Streptomyces kanamyceticus.

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