TRNA (pseudouridine54-N1)-methyltransferase (, TrmY, m1Psi methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine54 in tRNA S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA

This archaeal enzyme is specific for the 54 position.

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