The 1999–2000 season was the 85th season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England. The league consisted of four divisions.

Premier Division

The Premier Division consisted of 22 clubs, including 19 clubs from the previous season and three new clubs: Canvey Island, promoted as champions of Division One Hitchin Town, promoted as runners-up in Division One Farnborough Town, relegated from the Football Conference

Dagenham & Redbridge won the division and were promoted to the Football Conference. Aylesbury United, Boreham Wood and Walton & Hersham finished bottom of the table and relegated to Division One.

Before the start of the season Hampton changed name into Hampton & Richmond Borough.

League table

Stadia and locations

Club Stadium Aldershot Town Recreation Ground Aylesbury United Buckingham Road Basingstoke Town The Camrose Billericay Town New Lodge Boreham Wood Meadow Park Canvey Island Brockwell Stadium Carshalton Athletic War Memorial Sports Ground Chesham United The Meadow Enfield Meadow Park (groundshare with Boreham Wood) Dagenham & Redbridge Victoria Road Dulwich Hamlet Champion Hill Farnborough Town Cherrywood Road Gravesend & Northfleet Stonebridge Road Hampton & Richmond Borough Beveree Stadium Harrow Borough Earlsmead Stadium Hendon Claremont Road Heybridge Swifts Scraley Road Hitchin Town Top Field Slough Town Wexham Park St Albans City Clarence Park Thurrock Ship Lane Walton & Hersham The Sports Ground

Division One

Division One consisted of 22 clubs, including 17 clubs from the previous season and five new clubs:

Two clubs relegated from the Premier Division: Bishop's Stortford Bromley

Three clubs promoted from Division Two: Bedford Town Harlow Town Thame United

Croydon won the division and returned to the Premier Division. Grays Athletic and Maidenhead United also get a promotion. Chertsey Town and Leyton Pennant relegated to Division Two, while Leatherhead were reprieved after Hemel Hempstead were refused promotion from Division Two.

League table

Stadia and locations

Club Stadium Barton Rovers Sharpenhoe Road Bedford Town The Eyrie Bishop's Stortford Woodside Park Bognor Regis Town Nyewood Lane Braintree Town Cressing Road Bromley Hayes Lane Chertsey Town Alwyns Lane Croydon Croydon Sports Arena Grays Athletic New Recreation Ground Harlow Town Harlow Sportcentre Leatherhead Fetcham Grove Leyton Pennant Wadham Lodge Maidenhead United York Road Oxford City Marsh Lane Romford Sungate Staines Town Wheatsheaf Park Thame United Windmill Road Uxbridge Honeycroft Wealdstone White Lion (groundshare with Edgware Town) Whyteleafe Church Road Worthing Woodside Road Yeading The Warren

Division Two

Division Two consisted of 22 clubs, including 16 clubs from the previous season and six new clubs:

Three clubs relegated from Division One: Berkhamsted Town Molesey Wembley

Three clubs promoted from Division Three: Cheshunt Ford United Wingate & Finchley

Hemel Hempstead Town won the division, but were refused promotion due to ground grading. Northwood were promoted to Division One along with Ford United who get a second consecutive promotion. Wingate & Finchley finished in relegation zone along with Witham Town and Chalfont St Peter and returned to Division Three.

League table

Stadia and locations

Club Stadium Banstead Athletic Merland Rise Barking Mayesbrook Park Berkhamsted Town Broadwater Chalfont St Peter Mill Meadow Cheshunt Cheshunt Stadium Edgware Town White Lion Ford United Oakside Hemel Hempstead Town Vauxhall Road Horsham Queen Street Hungerford Town Bulpit Lane Leighton Town Bell Close Marlow Alfred Davis Memorial Ground Metropolitan Police Imber Court Molesey Walton Road Stadium Northwood Chestnut Avenue Tooting & Mitcham United Imperial Fields Wembley Vale Farm Windsor & Eton Stag Meadow Wingate & Finchley The Harry Abrahams Stadium Witham Town Spa Road Wivenhoe Town Broad Lane Wokingham Town Cantley Park

Division Three

Division Three consisted of 21 clubs, including 17 clubs from the previous season and four new clubs: Abingdon Town, relegated from Division Two Bracknell Town, relegated from Division Two Great Wakering Rovers, promoted as runners-up of the Essex Senior League Hertford Town, relegated from Division Two

Great Wakering Rovers debuted in the league and achieved promotion at the first attempt along with East Thurrock United and Tilbury. Southall resigned and joined the Combined Counties League.

League table

Stadia and locations

Club Stadium Abingdon Town Culham Road Aveley The Mill Field Bracknell Town Larges Lane Camberley Town Kroomer Park Clapton The Old Spotted Dog Ground Corinthian-Casuals King George's Field Croydon Athletic Keith Tuckey Stadium Dorking Meadowbank Stadium East Thurrock United Rookery Hill Egham Town The Runnymede Stadium Epsom & Ewell Merland Rise (groundshare with Banstead Athletic) Flackwell Heath Wilks Park Great Wakering Rovers Burroughs Park Hertford Town Hertingfordbury Park Hornchurch Hornchurch Stadium Kingsbury Town Avenue Park Lewes The Dripping PanSouthall Robert Parker Stadium Tilbury Chadfields Tring Town Pendley Ground Ware Wodson Park

See also Isthmian League 1999–2000 Northern Premier League 1999–2000 Southern Football League