Pseudobaptigenin synthase () is an enzyme with systematic name calycosin,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming). This enzyme catalyses two related chemical reactions:

Pseudobaptigenin synthase is a cytochrome P450 protein containing heme. It requires a partner cytochrome P450 reductase for functional expression. This uses nicotinamide adenine dinucleotide phosphate (NADPH). Pratensein, a derivative of calycosin with an extra hydroxy group, is similarly converted into 5-hydroxypseudobaptigenin:

The enzyme isolated from chickpea is part of the biosynthetic pathway to pterocarpans which act as phytoalexins.

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