Nitrilotriacetate monooxygenase () is an enzyme with systematic name nitrilotriacetate,FMNH2:oxygen oxidoreductase (glyoxylate-forming). This enzyme catalyses the following chemical reaction

The three substrates of this enzyme are nitrilotriacetic acid, reduced flavin adenine dinucleotide (FADH2), and oxygen. It products are iminodiacetic acid, FAD, glyoxylic acid, and water. It requires Mg2+.

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