3-ketosteroid 9alpha-monooxygenase (, KshAB, 3-ketosteroid 9alpha-hydroxylase) is an enzyme with systematic name androsta-1,4-diene-3,17-dione,NADH:oxygen oxidoreductase (9alpha-hydroxylating). This enzyme catalyses the following chemical reaction
androsta-1,4-diene-3,17-dione + NADH + H+ + O2 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O
3-Ketosteroid 9alpha-monooxygenase is involved in the cholesterol degradation in several bacterial pathogens.
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