Acetoin dehydrogenase (, acetoin dehydrogenase complex, acetoin dehydrogenase enzyme system, AoDH ES) is an enzyme with systematic name acetyl-CoA:acetoin O-acetyltransferase. This enzyme catalyses the following chemical reaction
This enzyme requires thiamine diphosphate and is involved in the catabolism of acetoin. It is widely present in bacteria and has been characterised from Alcaligenes eutrophus, Pelobacter carbinolicus, Clostridium magnum, Pseudomonas putida, and Bacillus subtilis.
See also Diacetyl reductase
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