Lukáš Trefil (; born 21 September 1988 in Prague) is a Czech canoeist. He won a bronze medal at the 2012 Summer Olympics and the 2016 Olympics in the K-4 1000 m event.

He has also been a World Champion and multiple time European champion in this event.

He first began canoeing in 1998 and began competing in 2005.

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