Holmsen is a Norwegian surname. Notable people with the surname include:
Andreas Holmsen (1906–1989), Norwegian historian Bjørn Holmsen (born 1948), Norwegian entrepreneur Borghild Holmsen (1865–1938), Norwegian pianist, music critic and composer Eivind Holmsen (1894–1990), Norwegian professional sports shooter Hanna Resvoll-Holmsen (1873–1943), Norwegian botanist Øivind Holmsen (1912–1996), Norwegian international footballer Sverre Holmsen (1906–1992), Swedish author