Amblyopinae is a subfamily of elongated mud-dwelling gobies commonly called eel gobies or worm gobies; it has been regarded as a subfamily of the family Gobiidae, while the 5th edition Fishes of the World classifies it as a subfamily of the family Oxudercidae. The members in the subfamily have two dorsal fins that are connected by a membranous structure and their eyes are highly reduced in size. They are usually pink, red, or purple in coloration.

Genera Currently, 15 genera in this subfamily are recognized: Amblyotrypauchen Hora, 1924 Biendongella Prokofiev, 2015 Prokofiev, A.M. (2015): An Overview of Gobies of the Subfamily Amblyopinae (Gobiidae) from the Western South China Sea (Vietnam and Indonesia) and Pacific Waters of the Philippines. Journal of Ichthyology, 55 (6): 783–791. Brachyamblyopus Bleeker, 1874 Caragobius H. M. Smith & Seale, 1906 Ctenotrypauchen Steindachner, 1867 Gymnoamblyopus Murdy & Ferraris, 2003 Karsten Murdy, 2002 Odontamblyopus Bleeker, 1874 Paratrypauchen Murdy, 2008 Pseudotrypauchen Hardenberg, 1931 Sovvityazius Prokofiev, 2015 Taenioides Lacépède, 1800 Trypauchen Valenciennes, 1837 Trypauchenichthys Bleeker, 1860 Trypauchenopsis Volz, 1903

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