Dusík (feminine: Dusíková) is a Czech surname. Dussek is a Germanised form of the surname, which appeared in the name of Jan Ladislav Dussek and his family. Notable people with the surname include:

František Josef Dusík (1765 – after 1816), composer and instrumentalist Jan Dusík (born 1975), Czech activist and politician Jan Ladislav Dussek, born Jan Václav Dusík (1760–1812), Czech composer and pianist Kateřina Veronika Anna Dusíková, also known as Veronika Dussek (1769–1833), Czech composer and pianist Olivia Buckley, also known as Olivia Dussek (1799–1847), English composer and harpist Sophia Dussek (1775 – c. 1831), Scottish composer, soprano and harpist

See also Dussek Campbell Ltd, formerly Dussek Brothers, British chemical company Dušek, similar Czech surname