Twelvetrees is an English surname with possible origin in Cornwall or in Sussex. Notable people with this surname include:
Billy Twelvetrees (born 1988), English rugby player Charles Twelvetrees (1872–1948), American artist and illustrator Harper Twelvetrees (1823–1881), British industrialist Helen Twelvetrees (1908–1958), American actress, daughter-in-law of Charles William Harper Twelvetrees (1848–1919), English-Australian geologist
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