Eot or EOT may refer to:
Science Equation of time Extraordinary optical transmission
Technology Electric overhead traveling crane Embedded OpenType, a font file format End-of-tape, a marker indicating the end of a magnetic tape End of Term Web Archive, project to preserve U.S. federal government websites during administration change End-of-Transmission character, a transmission control character Equivalent oxide thickness, of a semiconductor Eyes of Things, a computer vision project
Transport End-of-train device Engine order telegraph, a ship or submarine's speed control Massachusetts Executive Office of Transportation, a former government agency merged into the Massachusetts Department of Transportation
Education End of Term exams
Other uses Employee ownership trust Eot (island), in Micronesia Eotile language Greek National Tourism Organization ()