Mystery Room may referrer to:
Layton Brothers: Mystery Room, a 2012 puzzle adventure video game The Mystery Room in the Sears-Kay Ruin Fort in Carefree, Arizona The Mystery Room in the Arizona Biltmore Hotel in Phoenix, Arizona
See also Locked-room mystery, a type of crime seen in crime and detective fiction Room: The Mystery, a 2014 Indian thriller film The Mystery of the Secret Room, a 1945 children's novels by Enid Blyton