Venus Williams defeated Mary Pierce in the final, 6–4, 6–2 to win the women's singles tennis title at the 1999 Italian Open.

Martina Hingis was the defending champion, but lost in the semifinals to Williams in a rematch of the previous year's final.

Seeds The top eight seeds received a bye to the second round.

Draw

Finals

Top half

Section 1

Section 2

Bottom half

Section 3

Section 4

Qualifying

Seeds

Qualifiers

Lucky losers Tatiana Panova

Qualifying draw

First qualifier

Second qualifier

Third qualifier

Fourth qualifier

Fifth qualifier

Sixth qualifier

Seventh qualifier

Eighth qualifier

External links ITF tournament draws