Venus Williams defeated Mary Pierce in the final, 6–4, 6–2 to win the women's singles tennis title at the 1999 Italian Open.
Martina Hingis was the defending champion, but lost in the semifinals to Williams in a rematch of the previous year's final.
Seeds The top eight seeds received a bye to the second round.
Draw
Finals
Top half
Section 1
Section 2
Bottom half
Section 3
Section 4
Qualifying
Seeds
Qualifiers
Lucky losers Tatiana Panova
Qualifying draw
First qualifier
Second qualifier
Third qualifier
Fourth qualifier
Fifth qualifier
Sixth qualifier
Seventh qualifier
Eighth qualifier
External links ITF tournament draws