Oosthuizen is a South African surname, especially common among Afrikaners. Notable people with the name include:
Abrie Oosthuizen (fl. 1994–2013), South African politician Andries Oosthuizen (born c. 1954), South African golfer Caylib Oosthuizen (born 1989), South African rugby union player for the Tel Aviv Heat Coenie Oosthuizen (born 1989), South African rugby union player for Sale Sharks D. C. S. Oosthuizen (1926–1969; also known as Daantjie Oosthuizen), South African philosopher Etienne Oosthuizen (born 1992), South African rugby union player Etienne Oosthuizen (born 1994), South African rugby union player John Robert Oosthuizen (born 1987), South African javelin thrower Louis Oosthuizen (born 1982), South African professional golfer Ockie Oosthuizen (1955–2019), South African rugby union player Sarel Oosthuizen (1862–1900), Orange Free State Anglo-Boer War general Theo Oosthuizen (born 1964), South African rugby union player and coach Thomas Oosthuizen (born 1989), South African professional boxer
Also: Deon Oosthuysen (born 1963), South African rugby union player and coach
See also Oosthuizen, a village in the Dutch province of North Holland Oosthuizen (1789 ship) Oosthuizen's blue (Lepidochrysops oosthuizeni) – a species of butterfly in the family Lycaenidae