The 1964 Titleholders Championship was the 25th Titleholders Championship, held April 23–26 at Augusta Country Club in Augusta, Georgia.

Reigning champion Marilynn Smith successfully defended her title, one stroke ahead of runner-up Mickey Wright. The two had met in an 18-hole playoff the previous year which was decided on the final green. Wright had won the title in 1961 and 1962. It was the second and final major title for Smith.

Smith's total of 289 was two strokes better than the previous record of 291 set by Patty Berg in 1955. Her second round 66 (−6) on Friday helped her set several other records for the championship, including lowest round, lowest score after 36 holes at 139 (−5), and 54 holes at 216 (even). Her Friday inward nine score of 31 was also a record, by two strokes.

In the third round on Saturday, scores soared in the rain and cold wind. Smith's 77 (+5) kept the lead at an even-par 216 for 54 holes, three shots ahead of Wright.

Final leaderboard Sunday, April 26, 1964 Place Player Score To par Money ($)1 Marilynn Smith73-66-77-73=289+11,300 2 Mickey Wright74-70-75-71=290+21,0003 Betsy Rawls71-77-74-72=294+6800T4 Ruth Jessen77-72-74-73=296+8625 Judy Kimball71-72-78-75=296T6 Clifford Ann Creed75-69-79-74=297+9500 Shirley Englehorn75-75-73-74=2978 Mary Mills74-74-78-72=298+104009 Louise Suggs73-73-76-77=299+11350T10 Marlene Hagge75-72-78-75=300+12275 Sandra Haynie74-75-76-75=300 Judy Rankin75-73-76-76=300 Source:

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