Middleburg may refer to several places:
United States Middleburg, Florida Middleburg, Iowa Middleburg, Kentucky Middleburg, Carroll County, Maryland Middleburg, Washington County, Maryland Middleburg, a Dutch settlement on Long Island, now Elmhurst, Queens, New York, U.S. Middleburg, North Carolina Middleburg, Jefferson County, Ohio Middleburg, Logan County, Ohio Middleburg, Noble County, Ohio Middleburg, Seneca County, Ohio Middleburg, Pennsylvania Middleburg, Virginia
Other places Middleburg Island or Pulau Middelburg, Tambrauw, an island in West Papua, Indonesia
See also Middleburg Heights, Ohio Middleburg Township, Ohio Middleburgh (disambiguation) Middelburg (disambiguation) Middleberg (disambiguation) Middletown (disambiguation)