Putative tyrosine carboxypeptidase MATCAP2 is a protein that in humans is encoded by the MATCAP2 gene (previously KIAA0895). It is predicted to have carboxypeptidase activity (like its paralog MATCAP1), but this has yet to be confirmed.
__TOC__
Gene
Locus The MATCAP2 gene is located at 7p14.2. The genomic DNA is 65,976 base pairs long, while the longest mRNA that it produces is 4463 bases long.
It can be transcribed into 15 transcript variants, which in turn can produce 13 different isoforms of the protein.
Gene Neighborhood MATCAP2 is surrounded by the following genes on chromosome 7: EEPD1 MARK2P7 ANLN LOC111365168 AOAH
Size of gene The gene encoded for the MATCAP2 protein is 65,975 nucleotides long, from nucleotides 36324150 to 36390125, with seven exons.
mRNA There are ten different isoforms for MATCAP2. NP_001093895.1 EAW94064.1 NP_056129.2 NP_001186636.1 NP_001186635.1 XP_005249746.1 EAW94065.1 XP_024302470.1 NP_001186637.1 NP_001287885.1
Protein The longest protein isoform that is produced by the MATCAP2 gene is termed LOC23366 isoform 1 and is 520 amino acids long. The predicted molecular weight is 61kDa. Additionally, the theoretical isoelectric point is 10.
Amino acid composition MATCAP2 is a lysine and arginine semi-enriched protein. MATCAP2 is semi-enriched in positively charged lysine and arginine groups, and positively and negatively charged lysine, arginine, glutamic acid and aspartic acid groups. However, MATCAP2 is semi-depleted in non-polar alanine, glycine and proline groups.
The charge distribution analysis shows that there are no negative or mixed charge clusters. However, there is one positive charge cluster from amino acids 12 to 36.
Regions LOC23366 contains a protein domain of unknown function called DUF1704. It also contains a region of low complexity from position 120 to position 150 in the protein, and an arginine-rich area from position 12 to position 51.
Promoters Using ElDorado by Genomatrix, a promoter region sequence was found. The most likely promoter for MATCAP2 starts at 36389926 and goes to 36391149, with a length of 1224.
Post-translational modification MATCAP2 is predicted to undergo phosphorylation at several serines, threonines, and tyrosines throughout its structure. Phosphorylation at these sites is a form of gene regulation. Phosphorylation results in a conformational change in the structure of many enzymes and receptors. This causes them to become activated or deactivated.
Stem loops Using a database called Mfold, a stem-loop formation for the 5' UTR region shows that there is a lack of conservation, meaning there is some precedence that these stems and loops are not used for translation regulation. The ΔG value was -12.90 kcal/mol with three loops. Using the same database, a stem-loop formation for the 3' UTR region shows that there is conservation, meaning there is some precedence that they are used for translation regulation. The ΔG value was -636.80 kcal/mol.
Tertiary structure MATCAP2 has a tertiary structure with alpha helices and beta sheets.
Interacting proteins There are three proteins likely to be interacting proteins with MATCAP2. These proteins are ELAVL1, vata, and glym. These interactions have experimental evidence from the sources provided.
Expression MATCAP2 is most commonly found in the testis, however it also has a strong expression in the kidneys, adrenal glands, and brain.
Per Health State Using an EST profile from NCBI, MATCAP2 has strong expression in cervical tumors and bladder carcinoma.
Interacting proteins Using three different databases, three different interacting proteins were found. These include ELAVL1, VATA, and GLYM. There was experimental evidence for all three of these interacting proteins.
Homologs and orthologs MATCAP2 has over 228 orthologs. Orthologs have been found in mammals and eukaryotes. There are homologs in 9 species. The full list of organisms in which homologs have been found is given below.
Pan troglodytes Macaca mulatta Canis lupus familiaris Bos taurus Mus musculus Rattus Gallus gallus domesticus Danio rerio Anura
Paralogs MATCAP2 has 7 paralogs in Homo sapiens: Unnamed protein product Uncharacterized protein KIAA0895-like hCG28832, isoform CRA_a Hypothetical protein Unnamed protein product hCG38687, isoform CRA_a, partial hCG38687, isoform CRA_b
References