L27 or L-27 may refer to: L27 domain, a protein domain 60S ribosomal protein L27 Buick L27 engine, a V6 automobile engine Cessna L-27, a light utility aircraft of the U.S. Air Force , a submarine of the Royal Navy , a sloop of the Royal Navy Klemm L 27, a German training aircraft Lectionary 27, a medieval Greek manuscript