Mountain Brook may refer to:
Places in the United States Mountain Brook, Alabama, a city in southeastern Jefferson County, Alabama Mountain Brook School System, a public school system in Mountain Brook, Alabama Mountain Brook High School, a three-year public high school within the Mountain Brook School System Mountain Brook (New York), a tributary of the Little Delaware River near Bovina, New York
Livings things Duellmanohyla, or mountain brook frogs, a genus of frogs in the family Hylidae Ichthyomyzon greeleyi, also known as the mountain brook lamprey