An acidophile is an organism that thrives under highly acidic conditions.

Acidophile may also refer to:

Acidophile (histology), a particular staining pattern of cells and tissues when using haematoxylin and eosin stains

See also Acidofilia, 2002 album by Acid Drinkers Acidophil cell in the anterior pituitary Lactobacillus acidophilus, a species of bacteria Acidophobe, antonym of acidophile