Zweifel is a German surname. Notable people with the surname include:
Albert Zweifel (born 1949), former professional cyclo-cross cyclist Clint Zweifel (born 1973), American politician from Missouri David E. Zweifel (1934–2022), American diplomat Paul Zweifel (1848–1927), German gynaecologist and physiologist Richard G. Zweifel (1926–2019), American herpetologist Zweifel's frog (Rana zweifeli)