The Netherlands Football League Championship 1900–1901 was contested by fifteen teams participating in two divisions. The national champion would be determined by a play-off match featuring the winners of the eastern and western football division of the Netherlands. HVV Den Haag won this year's championship by beating Victoria Wageningen 2–1 in a decision match.

New entrants Eerste Klasse East: U.D. returned after one season of absence

Eerste Klasse West: Rapiditas Rotterdam returned after one season of absence Velocitas

Divisions

Eerste Klasse East

Eerste Klasse West

Championship play-off

Replay

HVV Den Haag won the championship.

References RSSSF Netherlands Football League Championships 1898-1954 RSSSF Eerste Klasse Oost RSSSF Eerste Klasse West