This is a list of accidents and incidents involving the Douglas DC-3 that have taken place since 1 January 2000, including aircraft based on the DC-3 airframe such as the Douglas C-47 Skytrain, Basler BT-67 and Lisunov Li-2. Military accidents are included; and hijackings and incidents of terrorism are covered, although acts of war are outside the scope of this list.

2000 17 March Douglas DC-3C C-FNTF of Points North Air Services crashed at Ennadai Lake Airport in Canada while attempting a go-around. Both crew died; the cause was found to be that the aircraft's centre of gravity was too far to the rear, possibly due to the cargo shifting in flight. Both crew members were found to have high levels of carboxyhaemoglobin in their blood. The flight had departed from Points North Landing Airport, Points North Landing, Saskatchewan.

20 July Douglas C-47A N54AA of Allied Air Freight suffered an engine failure on take-off from Grand Bahama International Airport in Freeport, Bahamas on a cargo flight to Nassau International Airport, Bahamas. The aircraft crashed while attempting to return to Grand Bahama and was destroyed. Both crew died.

2 September Basler BT-67 FAC1659 of the Fuerza Aérea Colombiana was destroyed when it flew into the high Mount Montezuma in Colombia; all seven on board died.

9 November In El Salvador, Basler BT-67 FAS119 of the Salvadoran Air Force was damaged beyond economic repair on landing at Los Comandos Airport, Los Comandos. The aircraft suffered brake failure, overran the runway and collided with a tree.

2001

23 January In the United States, Douglas DC-3C N19454 of Majestic Air Cargo was destroyed when it flew into Table Top Mountain at an altitude of while on a flight from Unalaska Airport to Ted Stevens Anchorage International Airport, Anchorage, Alaska. Investigation revealed that no flight plan had been filed, which delayed the reporting of the aircraft as overdue. Both crew members tested positive for drugs - the captain for cocaine and the first officer for amitriptyline and nortriptyline.

25 January RUTACA Airlines Flight 225, operated by Douglas DC-3C YV-224-C, crashed at Ciudad Bolívar in Venezuela; all 24 on board plus one person on the ground died. Another person on the ground was seriously injured. There were unconfirmed reports that a 25th person may have been on board the aircraft. The aircraft was on a non-scheduled domestic passenger flight from Tomás de Heres Airport, Ciudad Bolivar to Del Caribe "Santiago Mariño" International Airport, Porlamar and had developed an engine problem shortly after take-off.

15 March In the United States, Douglas C-47A N842MB of Jim Hankins Air Service made a successful emergency landing at Donalsonville Municipal Airport, Donalsonville, Georgia, following an inflight engine failure, fire and separation of the starboard engine. The aircraft was on a cargo flight from Panama City-Bay County International Airport, Panama City, Florida, to Southwest Georgia Regional Airport, Albany, Georgia, it was assessed as damaged beyond economic repair. The aircraft was transported to the Museum of Aviation, Warner Robins, Georgia, in August 2005.

4 April Douglas DC-3A N19BA of Roblex Aviation ditched in the sea off Luis Muñoz Marín International Airport in Carolina, Puerto Rico after suffering a double engine failure while on a local training flight. Both crew escaped. Damage to the aircraft was described as minor.

9 July In the United States, Douglas C-47A N3239T of the Valiant Air Command Warbird Museum was substantially damaged in a landing accident at Space Coast Regional Airport, Titusville, Florida. The aircraft was returning from an air show at Pope Air Force Base, Fayetteville, North Carolina, and had made a refuelling stop at Moore County Airport, Southern Pines, North Carolina. The aircraft was later repaired and returned to service.

December Air Katanga Douglas C-53 ZS-OJD was written off in a landing accident at Lubumbashi International Airport in the Democratic Republic of the Congo, after a delivery flight that originated in South Africa.

2002 21 May In the United States, Douglas DC-3A XB-JBR of Aero JBR ditched in Lake Casa Blanca, Texas, after a double engine failure while performing a touch-and-go at Laredo International Airport. It is reported that one of the engines suffered a propeller overspeed condition. All three crew escaped from the submerged aircraft.

2003

10 March Aero Modifications International (AMI) DC-3-65TP ZS-MFY of Rossair Contracts was substantially damaged in a landing accident at Rumbek Airport in Sudan due to encountering windshear. The aircraft was repaired, and flown out on 17 April 2003.

19 March Aerotaxi Flight 882, operated by Douglas DC-3C CU-T1192, was hijacked on a flight from Rafael Cabrera Airport, Nueva Gerona, Cuba to José Martí International Airport, Havana, Cuba. The aircraft landed in the United States at Key West International Airport, Key West, Florida, where the six hijackers were detained.

30 April In Colombia, Basler BT-67 PNC-0212 of the Servicio Aéreo de Policía was damaged beyond repair when it overran the runway at Aguas Claras Airport, Ocaña.

2 October A Douglas DC-3 operating an illegal flight for the Autodefensas Unidas de Colombia was destroyed by the Fuerza Aérea Colombiana at Bocas del Rosario in Colombia.

21 November Douglas C-47A ZS-BXF, operating South African Air Force Historic Flight Flight 668, was substantially damaged in a forced landing in South Africa after both engines failed shortly after take-off from Lanseria International Airport, Johannesburg, due to fuel mismanagement. The aircraft's destination was AFB Swartkop, Centurion. Repairs were carried out at OR Tambo International Airport, Kempton Park, Gauteng. The restored aircraft flew out on 10 November 2006.

30 December Douglas DC-3C N781T of Tol-Air Services was substantially damaged when the starboard undercarriage collapsed on landing at Cyril E. King Airport, Charlotte Amalie, United States Virgin Islands after a flight that originated at San Juan, Puerto Rico.

2004 March Fuerza Aérea Guatemalteca Basler BT-67 FAG-580 was written off when it overshot a runway on landing at an unknown location in Guatemala.

20 June In Colombia, Douglas C-49J HK-1212 of Viarco suffered an engine fire on take-off from Las Gaviotas Airport, Gaviotas and crashed into trees. The aircraft was on a non-scheduled domestic passenger flight to German Olano Airport, Puerto Carreño. All 20 on board escaped.

26 June In Russia, Lisunov Li-2T RA-1300K of FLA RF crashed at Zaozerye shortly after take-off from Myachkovo Airport, Moscow and was destroyed. The aircraft was operating a domestic non-scheduled passenger flight to Grabtsevo Airport, Kaluga. Two of the five people on board died.

23 July Fuerza Aérea Salvadoreña Basler BT-67 FAS117 was substantially damaged when the undercarriage collapsed on landing at El Jaguey in El Salvador.

13 August In the United States, Douglas DC-3 N22RB was written off at Orlando-Herndon Airport, Orlando, Florida, during Hurricane Charley when it was blown onto its back and smashed against a hangar.

15 October Douglas DC-3C HK-1504 of AeroVanguardia flew into an electricity line and crashed near Medellín, Colombia on approach to Enrique Olaya Herrera Airport while performing a domestic cargo flight from La Vanguardia Airport, Villavicencio to José María Córdova International Airport, Medellín. The flight had been diverted from its intended destination due to fog. The three crew died.

17 December Douglas DC-3 HK-2663 of Arall Colombia was substantially damaged when it overran the runway at Puerto Gaitán Airport in Puerto Gaitán, Colombia. The aircraft was on a domestic scheduled passenger flight from Barrancominas Airport to La Vanguardia Airport, Villavicencio when the starboard engine started vibrating and a diversion was made. Although substantially damaged, the aircraft was subsequently repaired and returned to service.

2005 6 June In Colombia, Douglas DC-3A HK-3462 of Transportes Aéreos del Ariari crashed near Miraflores shortly after take-off from Miraflores Airport when an engine caught fire. The aircraft was on a chartered domestic passenger flight to Jorge Enrique González Torres Airport, San José del Guaviare.

13 June In the United States, Douglas R4D-8 N3906J of Air Pony Express suffered an engine failure shortly after take-off from Fort Lauderdale Executive Airport, Fort Lauderdale, Florida, on an international cargo flight to Marsh Harbour Airport, Marsh Harbour, Bahamas. The aircraft was written off when it was put down on a street in the Coral Ridge Isles residential neighbourhood of Fort Lauderdale, hitting trees, parked cars and a building and subsequently catching fire. The engine that failed had had maintenance work performed immediately before the accident flight.

19 June In the Democratic Republic of the Congo, Douglas C-47B 9Q-CWI of Wimbi Dira Airways was damaged beyond repair when it suffered a groundloop on landing at Kabalo Airport, Kabalo. The aircraft ended up in a minefield and the wreckage remains in situ.

24 October In the United States, Douglas C-53 N7500A was substantially damaged at Opa-locka Airport, Florida during Hurricane Wilma.

2006 19 July Douglas DC-3C N782T of Tol-Air Services ditched into the sea off Charlotte Amalie, United States Virgin Islands after an engine failure shortly after take-off from Cyril E. King Airport. All four people on board escaped as the aircraft floated for about ten minutes before sinking. The aircraft now lies in of water and is a dive site.

2007

25 May In Colombia, Douglas DC-3C HK-3199 of AeroVanguardia was substantially damaged when it departed the runway at Acaricuara Airport, Acaricuara on landing after a flight from Jorge Enrique González Torres Airport, San José del Guaviare. The aircraft was repaired and returned to service.

31 July Douglas DC-3C HK-1149 of AeroVanguardia was substantially damaged when a forced landing was made in a rice field at Puerto Concordia in Colombia. The aircraft was on a scheduled domestic passenger flight from Jorge Enrique González Torres Airport, an José del Guaviare to La Vanguardia Airport, Villavicencio when an engine failed.

20 December Basler BT-67 C-FMKB of Kenn Borek Air was substantially damaged in a take-off accident at Mount Patterson, Antarctica when the take-off was attempted with insufficient speed for flight. Of the 12 people on board, only the co-pilot suffered minor injuries. Although both sets of undercarriage collapsed and the port wing was damaged, the aircraft was later repaired and returned to service.

2008 24 January Douglas DC-3C HK-2006 of Viarco was substantially damaged in a landing accident at Teresita Airport in Teresita, Colombia. The aircraft was operating a domestic non-scheduled passenger flight from Jorge Enrique González Torres Airport, San José del Guaviare. All 13 passengers and crew escaped, the aircraft was subsequently repaired and returned to service.

2009

4 January Basler BT-67 C-GEAJ of Antarctic Logistics Centre International crashed on landing at Tony Camp, Antarctica () after a flight from Novolazarevskaya Station.

4 February In the United States, Douglas DC-3-65/AR N834TP of the National Test Pilot School was substantially damaged in a take-off accident at Mojave Air and Space Port, Mojave, California. Both sets of undercarriage and the port engine were ripped off. The aircraft was on a local training flight. The accident was caused by an incorrectly set rudder trim.

18 February In Colombia, Basler BT-67 FAC1670 of the Fuerza Aérea Colombiana crashed near Captain Germán Olano Moreno Air Base, Palanquero on a local training flight. All five crew died.