List of restriction enzyme cutting sites: G–K

This article contains a list of the most studied restriction enzymes whose names start with G to K inclusive. It contains approximately 90 enzymes.

The following information is given:

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Restriction enzymes

Enzyme PDB code Source Recognition sequence Cut IsoschizomersGalI Gluconobacter albidus 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' GceI Gluconobacter cerinus 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' GceGLI Gluconobacter cerinus 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' GdiI Gluconobacter dioxyacetonicus 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, PceI, SarI, Sru30DI, SseBI, SteI, StuIGdiII Gluconobacter dioxyacetonicus 5' CGGCCR 3' GCCGGY 5' ---C GGCCR--- 3' 3' ---GCCGG Y--- 5' GstI Geobacillus stearothermophilus 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' GsuI Gluconobacter suboxydans H-15T 5' CTGGAG 3' GACCTC 5' ---CTGGAGN12NNNN --- 3' 3' ---GACCTCN12NN NN--- 5'

Enzyme PDB code Source Recognition sequence Cut IsoschizomersHacI Halococcus acetoinfaciens 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bme12I, Bsp67I, BstENII, CcyI, FnuEI, MgoI, NphI, Sau3AIHaeI Haemophilus aegyptius 5' WGGCCW 3' WCCGGW 5' ---WGG CCW--- 3' 3' ---WCC GGW--- 5' HaeII Haemophilus aegypticus 5' RGCGCY 3' YCGCGR 5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5' AccB2I, BfoI, Bme142I, Bsp143II, BstH2I, LpnIHaeIII Haemophilus aegypticus 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BsuRIHaeIV Haemophilus aegyptius 5' GAYN5RTC 3' CTRN5YAG 5' ---GAYN5RTCN8NNNNNN --- 3' 3' ---CTRN5YAGN8N NNNNN--- 5' HalI Hafnia alvei B6 5' GAATTC 3' CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5' HalII Hafnia alvei B6 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, AliAJI, Bsp63I, CfrA4I, CfuII, CstI, PstI, SflI, Srl5DI, Sst12IHapII Haemophilus aphrophilus 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' HgaIR.J Roberts, 1988, Nucleic Acids Res. 16(suppl):271 From p.213 Molecular Cell Biology 4th Edition by Lodish, Berk, Zipursky, Matsudaira, Baltimore and Darnell. Haemophilus gallinarum 5' GACGC 3' CTGCG 5' ---GACGCN4N NNNNN--- 3' 3' ---CTGCGN4NNNNNN --- 5' HgiI Herpetosiphon giganteus 3303 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AsuIII, BbiII, BstACI, HgiGI, HgiHII, Hin1I, PamIIHgiAI Herpetosiphon giganteus HP1023 5' GWGCWC 3' CWCGWG 5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5' Alw21I, AspHI, Bsh45I, BsiHKAI,

Bsm6I, HpyF46II, MspV281IHgiBI Herpetosiphon giganteus Hpg5 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Psp03I, VpaK11AIHgiCI Herpetosiphon giganteus Hpg9 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiHI, MspB4I, PfaAIHgiCII Herpetosiphon giganteus Hpg9 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme18I, BthAI, DsaIV, FspMSI, HgiHIII, Psp03I, VpaK11AIHgiCIII Herpetosiphon giganteus Hpg9 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' HgiDI Herpetosiphon giganteus Hpa2 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AsuIII, BbiII, BstACI, HgiGI, HgiI, Hin1I, PamIIHgiDII Herpetosiphon giganteus Hpa2 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' HgiEI Herpetosiphon giganteus Hpg24 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme216I, BthAI, DsaIV, ErpI, FspMSI, HgiHIII, VpaK11AIHgiGI Herpetosiphon giganteus Hpa1 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AosII, BbiII, BstACI, HgiI, HgiGI, Msp17I, PamIIHgiHI Herpetosiphon giganteus HP1049 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiCI, MspB4I, PfaAIHgiHII Herpetosiphon giganteus HP1049 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AosII, AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Msp17I, PamIIHgiHIII Herpetosiphon giganteus HP1049 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme216I, DsaIV, ErpI, HgiBI, Psp03I, VpaK11AI, VpaK11BIHgiJI Herpetosiphon giganteus HFS101 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11AIHgiJII Herpetosiphon giganteus HFS101 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' HgiS22I Herpetosiphon giganteus 5' CCSGG 3' GGSCC 5' ---CC SGG--- 3' 3' ---GGS CC--- 5' AhaI, AseII, AsuC2I, BcnI, CauII, Eco1831I, EcoHI, Kpn49kIIHhaI Haemophilus haemolyticus 5' GCGC 3' CGCG 5' ---GCG C--- 3' 3' ---C GCG--- 5' AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, Hin6I, HinP1I, SciNIHhaII Haemophilus haemolyticus 5' GANTC 3' CTNAG 5' ---G ANTC--- 3' 3' ---CTNA G--- 5' Hin1I Haemophilus influenzae RFL1 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, HgiI, HgiDI, HgiDI, Hsp92IHin1II Haemophilus influenzae RFL1 5' CATG 3' GTAC 5' ---CATG --- 3' 3' --- GTAC--- 5' Hin2I Haemophilus influenzae RFL2 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' Hin4I Haemophilus influenzae RFL4 5' GAYN5VTC 3' CTRN5BAG 5' ---GAYN5VTCN7NNNNNN --- 3' 3' ---CTRN5BAGN7N NNNNN--- 5' Hin6I Haemophilus influenzae RFL6 5' GCGC 3' CGCG 5' ---G CGC--- 3' 3' ---CGC G--- 5' AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, HhaI, HsoI, HspAI, SciNIHinJCI Haemophilus influenzae JC9 5' GTYRAC 3' CARYTG 5' ---GTY RAC--- 3' 3' ---CAR YTG--- 5' HinP1I 2FL3 Search PDBe Haemophilus influenzae P1 5' GCGC 3' CGCG 5' ---G CGC--- 3' 3' ---CGC G--- 5' BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HsoI, HspAI, SciNIHincII 2AUD Search PDBe Haemophilus influenzae Rc 5' GTYRAC 3' CARYTG 5' ---GTY RAC--- 3' 3' ---CAR YTG--- 5' HindII Haemophilus influenzae Rd 5' GTYRAC 3' CARYTG 5' ---GTY RAC--- 3' 3' ---CAR YTG--- 5' HindIII 2E52, Search PDBe Haemophilus influenzae Rd 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' HinfI Haemophilus influenzae Rf 5' GANTC 3' CTNAG 5' ---G ANTC--- 3' 3' ---CTNA G--- 5' HjaI Hyphomonas jannaschiana 5' GATATC 3' CTATAG 5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5' HpaI Haemophilus parainfluenzae 5' GTTAAC 3' CAATTG 5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5' HpaII Haemophilus parainfluenzae 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' HphI Haemophilus parahaemolyticus 5' GGTGA 3' CCACT 5' ---GGTGAN6NN --- 3' 3' ---CCACTN6N N--- 5' AsuHPI, SspD5IHpy8I Helicobacter pylori A 8-5 5' GTNNAC 3' CANNTG 5' ---GTN NAC--- 3' 3' ---CAN NTG--- 5' Hpy51I Helicobacter pylori 51 5' GTSAC 3' CASTG 5' --- GTSAC--- 3' 3' ---CASTG --- 5' Hpy99I 3GOX Helicobacter pylori J99 5' CGWCG 3' GCWGC 5' ---CGWCG --- 3' 3' --- GCWGC--- 5' Hpy178III Helicobacter pylori J178 5' TCNNGA 3' AGNNCT 5' ---TC NNGA--- 3' 3' ---AGNN CT--- 5' Hpy188I Helicobacter pylori J188 5' TCNGA 3' AGNCT 5' ---TCN GA--- 3' 3' ---AG NCT--- 5' Hpy188III Helicobacter pylori J188 5' TCNNGA 3' AGNNCT 5' ---TC NNGA--- 3' 3' ---AGNN CT--- 5' HpyAV Helicobacter pylori 26695 5' CCTTC 3' GGAAG 5' ---CCTTCN4NN --- 3' 3' ---GGAAGN4N N--- 5' HpyBI Helicobacter pylori Roberts 5' GTAC 3' CATG 5' ---GT AC--- 3' 3' ---CA TG--- 5' AfaI, Csp6I, CviQI, CviRII, PabI, PlaAII, RsaI, RsaNIHpyBII Helicobacter pylori Roberts 5' GTNNAC 3' CANNTG 5' ---GTN NAC--- 3' 3' ---CAN NTG--- 5' HpyCI Helicobacter pylori 5' GATATC 3' CTATAG 5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5' HpyC1I Helicobacter pylori 5' CCATC 3' GGTAG 5' ---CCATCNNNN N--- 3' 3' ---GGTAGNNNNN --- 5' HpyCH4I Helicobacter pylori CH4 5' CATG 3' GTAC 5' ---CATG --- 3' 3' --- GTAC--- 5' HpyCH4III Helicobacter pylori CH4 5' ACNGT 3' TGNCA 5' ---ACN GT--- 3' 3' ---TG NCA--- 5' HpyCH4IV Helicobacter pylori CH4 5' ACGT 3' TGCA 5' ---A CGT--- 3' 3' ---TGC A--- 5' HpyCH4V Helicobacter pylori CH4 5' TGCA 3' ACGT 5' ---TG CA--- 3' 3' ---AC GT--- 5' HpyF10VI Helicobacter pylori RFL10 5' GCN7GC 3' CGN7CG 5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' HpyF44III Helicobacter pylori RFL44 5' TGCA 3' ACGT 5' ---TG CA--- 3' 3' ---AC GT--- 5' HsoI Haemophilus somnus 2336 5' GCGC 3' CGCG 5' ---G CGC--- 3' 3' ---CGC G--- 5' AspLEI, BstHHI, CfoI, FnuDIII, Hin6I, HinP1I, HspAI, SciNIHsp92I Haemophilus sp. 92 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, HgiI, HgiDI, HgiHII, Hin1I, Hsp92IHsp92II Haemophilus sp. 92 5' CATG 3' GTAC 5' ---CATG --- 3' 3' --- GTAC--- 5' HspAI Haemophilus sp. A 5' GCGC 3' CGCG 5' ---G CGC--- 3' 3' ---CGC G--- 5' AspLEI, BspLAI, BstHHI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, SciNIHsuI Haemophilus suis 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'

Enzyme PDB code Source Recognition sequence Cut IsoschizomersItaI Ilyobcater tartaricus 5' GCNGC 3' CGNCG 5' ---GC NGC--- 3' 3' ---CGN CG--- 5'

Enzyme PDB code Source Recognition sequence Cut IsoschizomersKasI Kluyvera ascorbata 5' GGCGCC 3' CCGCGG 5' ---G GCGCC--- 3' 3' ---CCGCG G--- 5' Kaz48kI Klebsiella azeanae 5' RGGNCCY 3' YCCNGGR 5' ---RGGNC CY--- 3' 3' ---YC CNGGR--- 5' KoxII Klebsiella oxytoca 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' KpnI Klebsiella pneumoniae OK8 5' GGTACC 3' CCATGG 5' ---GGTAC C--- 3' 3' ---C CATGG--- 5' Acc65I, AhaB8I, Asp718I, SthIKpn2I Klebsiella pneumoniae RFL2 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' Aor13HI, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroIKpn378I Klebsiella pneumoniae 378 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Kpn2kI Klebsiella pneumoniae 2k 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' Kpn49kI Klebsiella pneumoniae 49k 5' GAATTC 3' CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5' Kpn49kII Klebsiella pneumoniae 49k 5' CCSGG 3' GGSCC 5' --- CCSGG--- 3' 3' ---GGSCC --- 5' AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Mgl14481I, NciIKspI Kluyvera sp. 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Ksp22I Kurthia sp. 22 5' TGATCA 3' ACTAGT 5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' AbaI, BclI, BsiQI, BspXII, BstT7I, FbaI, ParIKsp632I Kluyvera sp. 632 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' KspAI Kurthia sp. N88 5' GTTAAC 3' CAATTG 5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5' Kzo9I Kurthia zopfii 9 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bce243I, Bsp105I, BspFI, BstMBI, CpfI, LlaAI, NdeII, Sth368IKzo49I Kurthia zopfii 49 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11AI

Notes

List of restriction enzyme cutting sites: G–K

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