This article contains a list of the most studied restriction enzymes whose names start with O to R inclusive. It contains approximately 130 enzymes. The following information is given:

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Restriction enzymes

O

Enzyme PDB code Source Recognition sequence Cut IsoschizomersOfoI Oscillatoria foreaui A-1340 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I, PlaAI, PunAIOkrAI Oceanospirillum kriegii 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' BamHI, Bce751I, BnaI, Bsp98I, BstI, OkrAI, SolI, Uba4009IOliI Oceanospirillum linum 4-5D 5' CACN4GTG 3' GTGN4CAC 5' ---CACNN NNGTG--- 3' 3' ---GTGNN NNCAC--- 5' AleIOxaNI Oerskovia xanthineolytica N 5' CCTNAGG 3' GGANTCC 5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

P

Enzyme PDB code Source Recognition sequence Cut IsoschizomersPabI 2DVY Pyrococcus abyssi 5' GTAC 3' CATG 5' ---GTA C--- 3' 3' ---C ATG--- 5' AfaI, Csp6I, CviQI, HpyBI, HpyBI, PlaAII, RsaI, RsaNI,PacI Pseudomonas alcaligenes 5' TTAATTAA 3' AATTAATT 5' ---TTAAT TAA--- 3' 3' ---AAT TAATT--- 5' Pac25I Pseudomonas alcaligenes 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspAI, PspALI, XcyI, XmaI, XmaCIPaeI Pseudomonas aeruginosa 5' GCATGC 3' CGTACG 5' ---GCATG C--- 3' 3' ---C GTACG--- 5' PaeAI Pseudomonas aeruginosa 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' PaeBI Pseudomonas aeruginosa - 18 5' CCCGGG 3' GGGCCC 5' ---CCC GGG--- 3' 3' ---GGG CCC--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspALI, SmaI, TspMI, XcyI, XmaI, XmaCIPaeHI Pseudomonas aeruginosa 4148 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' PaeHII Pseudomonas aeruginosa 4148 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AliAJI, BspBI, BspMAI, CfuII, Ecl2zI, HalII, Srl5DI, Sst12I, XcpIPaeQI Pseudomonas aeruginosa Q2 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' PaeR7I Pseudomonas aeruginosa PA0303 pMG7 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliIPae2kI Pseudomonas aeruginosa 2k 5' AGATCT 3' TCTAGA 5' ---A GATCT--- 3' 3' ---TCTAG A--- 5' Pae5kI Pseudomonas aeruginosa 5k 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Pae14kI Pseudomonas aeruginosa 14k 5' CCGCGG 3' GGCGCC 5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5' Pae17kI Pseudomonas aeruginosa 17k 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' Pae18kI Pseudomonas aeruginosa 18k 5' AGATCT 3' TCTAGA 5' ---A GATCT--- 3' 3' ---TCTAG A--- 5' PagI Pseudomonas alcaligenes Sau 14-027 5' TCATGA 3' AGTACT 5' ---T CATGA--- 3' 3' ---AGTAC T--- 5' PalI Providencia alcalifaciens 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' PamI Phormidium ambiguum 5' TGCGCA 3' ACGCGT 5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, Pun14627IPamII Phormidium ambiguum 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AsuIII, BsaHI, BstACI, HgiDI, HgiGI, HgiHII, Hin1I, Hsp92I, Msp17IPanI Pseudomonas alkanolytica 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaIPasI Pseudomonas anguilliseptica RFL1 5' CCCWGGG 3' GGGWCCC 5' ---CC CWGGG--- 3' 3' ---GGGWC CC--- 5' — None in May 2010 —PauI Paracoccus alcaliphilus ZVK3-3 5' GCGCGC 3' CGCGCG 5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5' PauAI Phormidium autumnale 5' RCATGY 3' YGTACR 5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5' PauAII Phormidium autumnale 5' TTTAAA 3' AAATTT 5' ---TTT AAA--- 3' 3' ---AAA TTT--- 5' AhaIII, DraI, SruIPceI Planococcus citreus 55 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuIPciI Planococcus citreus SE-F45 5' ACATGT 3' TGTACA 5' ---A CATGT--- 3' 3' ---TGTAC A--- 5' PctI Planococcus citreus SM 5' GAATGC 3' CTTACG 5' ---GAATGCN --- 3' 3' ---CTTAC GN--- 5' Asp26HI, Asp27HI, Asp35HI BmaHI, BsaMI, BscCI, Mva1269IPde12I Paracoccus denitrificans 12 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbIPde133I Paracoccus denitrificans 133 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' Pde137I Paracoccus denitrificans 137 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' PdiI Pseudomonas diminuta Mck 33-321 5' GCCGGC 3' CGGCCG 5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5' PdmI Pseudomonas diminuta Auk 5-324 5' GAAN4TTC 3' CTTN4AAG 5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, MroXI, XmnIPfaAI Phormidium favosum 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BanI, BbvBI, BshNI, Eco64I, HgiCI, HgiHI, MspB4IPfaAII Phormidium favosum 5' CATATG 3' GTATAC 5' ---CA TATG--- 3' 3' ---GTAT AC--- 5' PfaAIII Phormidium favosum 5' GCATGC 3' CGTACG 5' ---GCATG C--- 3' 3' ---C GTACG--- 5' PfeI Psychrobacter faecalis RFL1 5' GAWTC 3' CTWAG 5' ---G AWTC--- 3' 3' ---CTWA G--- 5' Pfl8I Pseudomonas fluorescens 8 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AliAJI, BspBI, BsuBI, CflI, CfrA4I, PaePI, Pfl21I, PstI, SflII, Sst12IPfl21I Pseudomonas sp. 21 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' Pfl23II Pseudomonas fluorescens RFL23 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5' Pfl27I Pseudomonas fluorescens RFL27 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5' PflBI Pseudomonas fluorescens type B 5' CCAN5TGG 3' GGTN5ACC 5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflMI, Van91IPflFI Pseudomonas fluorescens biotype F 5' GACNNNGTC 3' CTGNNNCAG 5' ---GACN NNGTC--- 3' 3' ---CTGNN NCAG--- 5' AspI, AtsI, PsyI, TelI, Tth111IPflKI Pseudomonas fluorescens 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' PflMI Pseudomonas fluorescens 5' CCAN5TGG 3' GGTN5ACC 5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, Van91IPfoI Pseudomonas fluorescens biovar 126 5' TCCNGGA 3' AGGNCCT 5' ---T CCNGGA--- 3' 3' ---AGGNCC T--- 5' PgaI Pseudomonas gladioli B4 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BbvAII, BliAI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144IPhaI Pasteurella haemolytica 5' GCATC 3' CGTAG 5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5' PhoI Pyrococcus horikoshii OT3 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' PinAI Pseudomonas inequalis 5' ACCGGT 3' TGGCCA 5' ---A CCGGT--- 3' 3' ---TGGCC A--- 5' AgeI, AsiAI, AsiGI, BshTI CsiAI, CspAIPinBI Phormidium inundatum 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' EcoT22I, BfrBI, Mph1103I, NsiI, Ppu10I, SepI, SspD5II, Zsp2IPinBII Phormidium inundatum 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' Aor13HI, BlfI, Bsp13I, BspEI, Bsu23I, Kpn2I, PinBII, PtaIPlaI Phormidium lapideum 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' PlaII Phormidium lapideum 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, SfuI, SspRFIPlaAI Phormidium laminosum 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' AquI, BcoI, Bse15I, BspLU4I, Eco27kI, Nli3877I, OfoI, PunAIPlaAII Phormidium laminosum 5' GTAC 3' CATG 5' ---GT AC--- 3' 3' ---CA TG--- 5' AfaI, Csp6I, CviQI, CviRII, HpyBI, PabI, RsaI, RsaNI,PleI Pseudomonas lemoignei 5' GAGTC 3' CTCAG 5' ---GAGTCNNNN N--- 3' 3' ---CTCAGNNNNN --- 5' Ple19I Pseudomonas lemoignei 19 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa22MI, BspCI, ErhB9I, MvrI, Psu161I, PvuI, RshI, XorIIPmaCI Pseudomonas maltophilia CB50P 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmlI, PspCIPmeI Pseudomonas mendocina 5' GTTTAAAC 3' CAAATTTG 5' ---GTTT AAAC--- 3' 3' ---CAAA TTTG--- 5' MssIPme55I Pseudomonas mendocina 55 5' AGGCCT 3' TCCGGA 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuIPmlI Pseudomonas maltophilia 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PspCIPovII Proteus vulgaris 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' PpaAI Phormidium papyraceum 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, Csp68KII, SfuI, SspRFI, SviIPpaAII Phormidium papyraceum 5' TCGA 3' AGCT 5' ---T CGA--- 3' 3' ---AGC T--- 5' PpeI Phormidium persicinum 5' GGGCCC 3' CCCGGG 5' ---GGGCC C--- 3' 3' ---C CCGGG--- 5' ApaI, Bsp120I, PspOMIPpiI Pseudomonas putida Jo 4-731 5' GAACN5CTC 3' CTTGN5GAG 5' ---GAACN5CTCN7NNNNNN --- 3' 3' ---CTTGN5GAGN7N NNNNN--- 5' PpsI Pseudomonas pseudoalcaligenes PL 5' GAGTC 3' CTCAG 5' ---GAGTCNNNN N--- 3' 3' ---CTCAGNNNNN --- 5' Ppu10I Pseudomonas putida RFL10 5' ATGCAT 3' TACGTA 5' ---A TGCAT--- 3' 3' ---TACGT A--- 5' BfrBI, Csp68KII, EcoT22I, Mph1103I, NsiI, SepI, SspD5IIPpu111I Pseudomonas putida P111 5' GAATTC 3' CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5' PpuAI Pseudomonas putida A1 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5' PpuMI Pseudomonas putida M 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5' PpuXI Pseudomonas putida X 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5' PshAI Plesiomonas shigelloides 319-73 5' GACN4GTC 3' CTGN4CAG 5' ---GACNN NNGTC--- 3' 3' ---CTGNN NNCAG--- 5' PshBI Plesiomonas shigelloides TPS970 5' ATTAAT 3' TAATTA 5' ---AT TAAT--- 3' 3' ---TAAT TA--- 5' AseI, AsnI, BpoAI, Sru4DI, VspIPsiI Pseudomonas sp. SE-G49 5' TTATAA 3' AATATT 5' ---TTA TAA--- 3' 3' ---AAT ATT--- 5' Psp03I Phormidium sp. 5' GGWCC 3' CCWGG 5' ---GGWC C--- 3' 3' ---C CWGG--- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11AIPsp5II Pseudomonas sp. RFL5 5' RGGWCCY 3' YCCWGGR 5' ---RG GWCCY--- 3' 3' ---YCCWG GR--- 5' Psp6I Pseudomonas sp. B6 5' CCWGG 3' GGWCC 5' --- CCWGG--- 3' 3' ---GGWCC --- 5' AeuI, AglI, ApaORI, Bse17I, EcoRII, Fsp1604I, SspAI, Sth117IPsp23I Pseudomonas sp. 23 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' ApiI, BspBI, CflI, MhaAI, Pfl21I, PstI, SalPI, Sst12I, YenIPsp1406I Pseudomonas pseudoalcaligenes RFL1406 5' AACGTT 3' TTGCAA 5' ---AA CGTT--- 3' 3' ---TTGC AA--- 5' AclIPspAI Pseudomonas sp. 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, SmaI, TspMI, XcyI, XmaI, XmaCIPspALI Pseudomonas sp. AL1637 5' CCCGGG 3' GGGCCC 5' ---CCC GGG--- 3' 3' ---GGG CCC--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspALI, SmaI, TspMI, XcyI, XmaI, XmaCIPsp124BIPseudomonas sp. 124B 5' GAGCTC 3' CTCGAG 5' ---GAGCT C--- 3' 3' ---C TCGAG--- 5' PspCIPseudomonas sp. C 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PmlIPspEIPseudomonas sp. E 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstPI, Eci125I, Eco91I, EcoO65I, EcoO128IPspGI 3BM3Pyrococcus sp. GI-H 5' CCWGG 3' GGWCC 5' --- CCWGG--- 3' 3' ---GGWCC --- 5' AeuI, AjnI, AorI, Bse17I, EcoRII, Fsp1604I, Psp6I, SspAI, Sth117IPspLIPseudomonas sp. L 5' CGTACG 3' GCATGC 5' ---C GTACG--- 3' 3' ---GCATG C--- 5' PspN4I Pseudomonas sp. N4 5' GGNNCC 3' CCNNGG 5' ---GGN NCC--- 3' 3' ---CCN NGG--- 5' AspNI, BscBI, BmiI, BspLI,