List of restriction enzyme cutting sites: T–Z

This article contains a list of the most studied restriction enzymes whose names start with T to Z inclusive. It contains approximately 70 enzymes.

The following information is given:

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Restriction enzymes

Enzyme PDB code Source Recognition sequence Cut IsoschizomersTaaI Thermus aquaticus Vn 4-311 5' ACNGT 3' TGNCA 5' ---ACN GT--- 3' 3' ---TG NCA--- 5' TaiI Thermus aquaticus cs1-331 5' ACGT 3' TGCA 5' ---ACGT --- 3' 3' --- TGCA--- 5' TaqI Thermus aquaticus 5' TCGA 3' AGCT 5' ---T CGA--- 3' 3' ---AGC T--- 5' TaqII Thermus aquaticus YTI 5' GACCGA 3' CTGGCT 5' ---GACCGAN8NNN --- 3' 3' ---CTGGCTN8N NN--- 5' Taq52I Thermus aquaticus YS52 5' GCWGC 3' CGWCG 5' ---G CWGC--- 3' 3' ---CGWC G--- 5' AceI, ApeKI, SuiI, TseITaqXI Thermus aquaticus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, MvaI, SleI, SspAITasI Thermus aquaticus Vn 4-211 5' AATT 3' TTAA 5' --- AATT--- 3' 3' ---TTAA --- 5' TatI Thermus aquaticus CBA1-331 5' WGTACW 3' WCATGW 5' ---W GTACW--- 3' 3' ---WCATG W--- 5' TauI Thermus aquaticus 5' GCSGC 3' CGSCG 5' ---GCSG C--- 3' 3' ---C GSCG--- 5' TelI Tolypothrix elabens 5' GACNNNGTC 3' CTGNNNCAG 5' ---GACN NNGTC--- 3' 3' ---CTGNN NCAG--- 5' AspI, AtsI, PflFI, PsyI, Tth111ITfiI Thermus filiformis 5' GAWTC 3' CTWAG 5' ---G AWTC--- 3' 3' ---CTWA G--- 5' ThaI Thermoplasma acidophilum 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FalII, FnuDIITliI Thermococcus litoralis 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, StrI, XhoITru1I Thermus ruber RFL1 5' TTAA 3' AATT 5' ---T TAA--- 3' 3' ---AAT T--- 5' Tru9I Thermus ruber 9 5' TTAA 3' AATT 5' ---T TAA--- 3' 3' ---AAT T--- 5' Tru201I Thermus ruber 201 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' TscI Thermus sp. 491A 5' ACGT 3' TGCA 5' ---ACGT --- 3' 3' --- TGCA--- 5' TseI Thermus sp. 93170 5' GCWGC 3' CGWCG 5' ---G CWGC--- 3' 3' ---CGWC G--- 5' AceI, ApeKI, SuiI, Taq52ITsp1I Thermus sp. 1 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5' Tsp32I Thermus sp. 32 5' TCGA 3' AGCT 5' ---T CGA--- 3' 3' ---AGC T--- 5' Tsp32II Thermus sp. 32 5' TCGA 3' AGCT 5' ---T CGA--- 3' 3' ---AGC T--- 5' Tsp45I Thermus sp. YS45 5' GTSAC 3' CASTG 5' --- GTSAC--- 3' 3' ---CASTG --- 5' Tsp49I Thermus sp. 5' ACGT 3' TGCA 5' ---ACGT --- 3' 3' --- TGCA--- 5' Tsp509I Thermus sp. 5' AATT 3' TTAA 5' --- AATT--- 3' 3' ---TTAA --- 5' TspBI Thermophilic sp. 5' CCRYGG 3' GGYRCC 5' ---C CRYGG--- 3' 3' ---GGYRC C--- 5' Tsp4CI Thermus sp. 4C 5' ACNGT 3' TGNCA 5' ---ACN GT--- 3' 3' ---TG NCA--- 5' TspDTI Thermus sp. DT 5' ATGAA 3' TACTT 5' ---ATGAAN8NNN --- 3' 3' ---TACTTN8N NN--- 5' — None in May 2010 —TspEI Thermus sp. 1E 5' AATT 3' TTAA 5' --- AATT--- 3' 3' ---TTAA --- 5' Tsp8EI Thermus sp. 8E 5' GCCN5GGC 3' CGGN5CCG 5' ---GCCNNNN NGGC--- 3' 3' ---CGGN NNNNCCG--- 5' TspGWI Thermus sp. GW 5' ACGGA 3' TGCCT 5' ---ACGGAN8NNN --- 3' 3' ---TGCCTN8N NN--- 5' TspMI = UthSI Unidentified thermophile 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, SmaI, XcyI, XmaI, XmaCITspRI Thermus sp. R 5' CASTGNN 3' GTSAC 5' ---CASTGNN --- 3' 3' --- GTSACNN--- 5' Tth111I Thermus thermophilus 111 5' GACNNNGTC 3' CTGNNNCAG 5' ---GACN NNGTC--- 3' 3' ---CTGNN NCAG--- 5' AspI, AtsI, PflFI, PsyI, TelITth111II Thermus thermophilus 111 5' CAARCA 3' GTTYGT 5' ---CAARCAN8NNN --- 3' 3' ---GTTYGTN8N NN--- 5' TthHB8I Thermus thermophilus HB8 5' TCGA 3' AGCT 5' ---T CGA--- 3' 3' ---AGC T--- 5'

Enzyme PDB code Source Recognition sequence Cut IsoschizomersUba4009I Unidentified bacterium A 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, RspLKII, SolIUba153AI Unidentified bacterium 153A 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' UbaM39I Unidentified bacterium M39 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' UnbI Unidentified bacterium #8 5' GGNCC 3' CCNGG 5' --- GGNCC--- 3' 3' ---CCNGG --- 5' AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV, UnbIUur960I Ureaplasma urealyticum 960 5' GCNGC 3' CGNCG 5' ---GC NGC--- 3' 3' ---CGN CG--- 5'

Enzyme PDB code Source Recognition sequence Cut IsoschizomersVan91I Vibrio anguillarum RFL91 5' CCAN5TGG 3' GGTN5ACC 5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMIVha464I Vibrio harveyi 464 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' VneI Vibrio nereis 18 5' GTGCAC 3' CACGTG 5' ---G TGCAC--- 3' 3' ---CACGT G--- 5' Alw44I, ApaLI, SnoIVpaK32I Vibrio parahaemolyticus 4387-61 5' GCTCTTC 3' CGAGAAG 5' ---GCTCTTCN NNN--- 3' 3' ---CGAGAAGNNNN --- 5' VpaK11AI Vibrio parahaemolyticus 1743 5' GGWCC 3' CCWGG 5' --- GGWCC--- 3' 3' ---CCWGG --- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11BIVpaK11BI Vibrio parahaemolyticus 1743-1 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BsrAI, CauI, EagMI, FdiI, HgiBI, HgiJI, SinI, VpaK11AIVspI Vibrio sp. 343 5' ATTAAT 3' TAATTA 5' ---AT TAAT--- 3' 3' ---TAAT TA--- 5' AseI, AsnI, BpoAI, PshBI, Sru4DI

Enzyme PDB code Source Recognition sequence Cut IsoschizomersXagI Xanthobacter agilis Vs 18-132 5' CCTN5AGG 3' GGAN5TCC 5' ---CCTNN NNNAGG--- 3' 3' ---GGANNN NNTCC--- 5' XapI Xanthomonas ampelina Slo 51-021 5' RAATTY 3' YTTAAR 5' ---R AATTY--- 3' 3' ---YTTAA R--- 5' AcsI, ApoI, CfaI, FsiIXbaI Xanthomonas badrii 5' TCTAGA 3' AGATCT 5' ---T CTAGA--- 3' 3' ---AGATC T--- 5' XcaI Xanthomonas campestris 5' GTATAC 3' CATATG 5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' XceI Xanthomonas campestris Ast 40-024 5' RCATGY 3' YGTACR 5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5' XciI Xanthomonas citri 5' GTCGAC 3' CAGCTG 5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5' XcmI Xanthomonas campestris 5' CCAN9TGG 3' GGTN9ACC 5' ---CCANNNNN NNNNTGG--- 3' 3' ---GGTNNNN NNNNNACC--- 5' XcyI Xanthomonas cyanopsidis 13D5 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XmaI, XmaCIXhoI Xanthomonas holcicola 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI, PanI, Sau3239I, Sfr274I, TliI, XpaIXhoII Xanthomonas holcicola 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' XmaI Xanthomonas malvacearum 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, TspMI, XcyI, XmaCIXmaIII Xanthomonas malvacearum 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16IXmaCI Xanthomonas malvacearum C 5' CCCGGG 3' GGGCCC 5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PaeBI, Pac25I, PspAI, TspMI, XcyI, XmaIXmaJI Xanthomonas maltophilia Jo 85-025 5' CCTAGG 3' GGATCC 5' ---C CTAGG--- 3' 3' ---GGATC C--- 5' AspA2I, AvrII, AvrBII, BlnI, BspA2IXmiI Xanthomonas maltophilia Jo 21-021 5' GTMKAC 3' CAKMTG 5' ---GT MKAC--- 3' 3' ---CAKM TG--- 5' AccI, FblIXmnI Xanthomonas manihotis 7AS1 5' GAAN4TTC 3' CTTN4AAG 5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, MroXI, PdmIXorII Xanthomonas oryzae 5' CGATCG 3' GCTAGC 5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5' Afa16RI, BspCI, EagBI, ErhB9I, MvrI, Ple19I, PvuI, RshIXpaI Xanthomonas papavericola 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BssHI, MavI, PanI, SauLPII, Sbi68I, Sol10179I, StrI, XhoIXspI Xanthomonas sp. YK1 5' CTAG 3' GATC 5' ---C TAG--- 3' 3' ---GAT C--- 5'

Enzyme PDB code Source Recognition sequence Cut IsoschizomersYenI Yersinia enterocolitica 08 A2635 5' CTGCAG 3' GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' Asp713I, BsuBI, CfrA4I, Ecl37kI, Psp23I, PstI, SalPI, SflI, Sst12I

Enzyme PDB code Source Recognition sequence Cut IsoschizomersZanI Zymomonas anaerobia 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' ApaORI, BseBI, BspNI, BstNI, Bst2UI, CthII, EcoRII, MvaI, SspAIZhoI Zymomonas holcicola 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BbvAII, BscI, BspJI, ClaI, LcaI, PgaI, SpmI, Ssp27144IZraI Zoogloea ramigera 11 5' GACGTC 3' CTGCAG 5' ---GAC GTC--- 3' 3' ---CTG CAG--- 5' AatII, Ssp5230IZrmI Zoogloea ramigera SCA 5' AGTACT 3' TCATGA 5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5' Acc113I, AssI, BmcAI, Bpa34I, DpaI, Eco255I, RflFII, ScaIZsp2I Zoogloea sp. 2 5' ATGCAT 3' TACGTA 5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5' BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II

Notes

List of restriction enzyme cutting sites: T–Z

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