This article contains a list of the most studied restriction enzymes whose names start with Bst to Bv inclusive. It contains approximately 200 enzymes.
The following information is given:
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Restriction enzymes
Bst
Enzyme PDB code Source Recognition sequence Cut IsoschizomersBstI Bacillus stearothermophilus 1503-4R 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, Nsp29132IIBst1I Bacillus stearothermophilus 1 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AglI, BseBI, Bse17I, BstNI, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXIBst2I Bacillus stearothermophilus 2 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AglI, BseBI, Bse17I, BstNI, BstOI, Bst2UI, CthII, EcoRII, MvaIBst6I Bacillus stearothermophilus 6 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' Bst11I Bacillus stearothermophilus 11 5' ACTGG 3' TGACC 5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5' Bst12I Bacillus stearothermophilus 12 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst71I, BstV1IBst19I Bacillus stearothermophilus 19 5' GCATC 3' CGTAG 5' ---GCATCNNNN NN--- 3' 3' ---CGTAGNNNNNN --- 5' Bst19II Bacillus stearothermophilus 19 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAIBst28I Bacillus stearothermophilus 28 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BciBI, BdiI, Bli41I, Bsa29I, Bsp106I, BspDI, LplI, SpmI, ZhoIBst38I Bacillus stearothermophilus 38 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AorI, BseBI, Bse17I, BsiLI, BspNI, BstOI, Bst2UI, EcoRII, MvaIBst40I Bacillus stearothermophilus 40 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' Bst71I Bacillus stearothermophilus 71 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, BstV1IBst98I Bacillus stearothermophilus C98 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' Bst100I Bacillus stearothermophilus 100 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleIBst1107I Bacillus stearothermophilus RFL1107 5' GTATAC 3' CATATG 5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' BstACI Bacillus stearothermophilus AC 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, HgiDI, Hsp92IBstAPI Bacillus stearothermophilus AP 5' GCAN4NTGC 3' CGTN4NACG 5' ---GCANNNN NTGC--- 3' 3' ---CGTN NNNNACG--- 5' ApaBIBstAUI Bacillus stearothermophilus AU 5' TGTACA 3' ACATGT 5' ---T GTACA--- 3' 3' ---ACATG T--- 5' AauI, BsmRI, Bsp1407I, BsrGI, Ssp4800I, SspBIBstBI Bacillus stearothermophilus B225 5' TTCGAA 3' AAGCTT 5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, LspI, NspV, SspRFIBst2BI Bacillus stearothermophilus 2B 5' CACGAG 3' GTGCTC 5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5' BstBAI Bacillus stearothermophilus BA 5' YACGTR 3' RTGCAY 5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5' BstBSI Bacillus stearothermophilus BS 5' GTATAC 3' CATATG 5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' BstB7SI Bacillus stearothermophilus B7S 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' BstBS32I Bacillus stearothermophilus BS32 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BstBZ153I Bacillus stearothermophilus BZ153 5' GCGCGC 3' CGCGCG 5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5' Bst4CI Bacillus stearothermophilus 4C 5' ACNGT 3' TGNCA 5' ---ACN GT--- 3' 3' ---TG NCA--- 5' BstC8I Bacillus stearothermophilus C8 5' GCNNGC 3' CGNNCG 5' ---GCN NGC--- 3' 3' ---CGN NCG--- 5' BstD102I Bacillus stearothermophilus D102 5' CCGCTC 3' GGCGAG 5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5' AccBSI, BsrBI, Bst31NI, MbiIBstDEI Bacillus stearothermophilus DE 5' CTNAG 3' GANTC 5' ---C TNAG--- 3' 3' ---GANT C--- 5' BstDSI Bacillus stearothermophilus DS 5' CCRYGG 3' GGYRCC 5' ---C CRYGG--- 3' 3' ---GGYRC C--- 5' BstEII Bacillus stearothermophilus ET 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, BstPI, BstT9I, BstT10I, EcaI, Eco91I, EcoO65I, NspSAII, PspEIBstENI Bacillus stearothermophilus EN 5' CCTN4NAGG 3' GGAN4NTCC 5' ---CCTNN NNNAGG--- 3' 3' ---GGANNN NNTCC--- 5' BstENII Bacillus stearothermophilus EN 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAIBstEZ359I Bacillus stearothermophilus EZ359 5' GTTAAC 3' CAATTG 5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5' BstFI Bacillus stearothermophilus FH58 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' BstF5I Bacillus stearothermophilus F5 5' GGATG 3' CCTAC 5' ---GGATGNN --- 3' 3' ---CCTAC NN--- 5' BstFNI Bacillus stearothermophilus 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, BceBI, BepI, Bpu95I, BstUI, Csp68KVI, FauBII, MvnI, SelIBstFZ438I Bacillus stearothermophilus FZ438 5' CCCGC 3' GGGCG 5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5' BstGZ53I Bacillus stearothermophilus GZ53 5' CGTCTC 3' GCAGAG 5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5' BstH2I Bacillus stearothermophilus H2 5' RGCGCY 3' YCGCGR 5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5' AccB2I, BfoI, Bme142I, Bsp143II, HaeII, LpnIBstH9I Bacillus stearothermophilus H9 5' GGATC 3' CCTAG 5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' AclWI, AlwI, BinI, BpuFI, BspPI, Bst31TI, BthII, Bth617I, EacIBstHHI Bacillus stearothermophilus HH 5' GCGC 3' CGCG 5' ---GCG C--- 3' 3' ---C GCG--- 5' AspLEI, BspLAI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNIBstHPI Bacillus stearothermophilus HP 5' GTTAAC 3' CAATTG 5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5' BstHZ55I Bacillus stearothermophilus HZ55 5' CCAN6TGG 3' GGTN6ACC 5' ---CCANNNNN NTGG--- 3' 3' ---GGTN NNNNNACC--- 5' BstIZ316I Bacillus stearothermophilus IZ316 5' CACNNNGTG 3' GTGNNNCAC 5' ---CACNNN GTG--- 3' 3' ---GTG NNNCAC--- 5' AdeI, DraIIIBstJZ301I Bacillus stearothermophilus JZ301 5' CTNAG 3' GANTC 5' ---C TNAG--- 3' 3' ---GANT C--- 5' BstKTI Bacillus stearothermophilus KT 5' GATC 3' CTAG 5' ---GAT C--- 3' 3' ---C TAG--- 5' AspMDI, Bsp2095I, Bst19II, BspKT6I, DpnII, MboI, NmeCI, SsiAIBstM6I Bacillus stearothermophilus M6 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, Fsp1604I, MvaIBstMAI Bacillus stearothermophilus MA 5' GTCTC 3' CAGAG 5' ---GTCTCN NNNNN--- 3' 3' ---CAGAGNNNNN N--- 5' Alw26I, BcoDI, BscQII, BsmAI, BsoMAIBstMBI Bacillus stearothermophilus MB 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368IBstMCI Bacillus stearothermophilus MC 5' CGRYCG 3' GCYRGC 5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5' BstMWI Bacillus stearothermophilus MW 5' GCN7GC 3' CGN7CG 5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' BstMZ611I Bacillus stearothermophilus MZ611 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' BstNI Bacillus stearothermophilus 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, BciBII, BptI, Bst1I, BstOI, Bst2UI, Fsp1604I, SniI, Sth117IBst31NI Bacillus stearothermophilus 31N 5' CCGCTC 3' GGCGAG 5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5' AccBSI, BsrBI, BstD102I, MbiIBstNSI Bacillus stearothermophilus 1161NS 5' RCATGY 3' YGTACR 5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5' BstNZ169I Bacillus stearothermophilus NZ169 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BanIII, BciBI, BdiI, Bli41I, Bsa29I, BspDI, LplI, Rme21I, SpmI, ZhoIBstOI Bacillus stearothermophilus O22 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, ApyI, BciBII, BptI, Bst1I, Bst2UI, Fsp1604I, SniI, Sth117IBstOZ616I Bacillus stearothermophilus OZ616 5' GGGAC 3' CCCTG 5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' BstPI Bacillus stearothermophilus 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstT9I, EcaI, Eco91I, EcoO65I, NspSAII, PspEIBstPAI Bacillus stearothermophilus PA 5' GACN4GTC 3' CTGN4CAG 5' ---GACNN NNGTC--- 3' 3' ---CTGNN NNCAG--- 5' BstPZ418I 5' GGATG 3' CCTAC 5' ---GGATGN8N NNNN--- 3' 3' ---CCTACN8NNNNN --- 5' BstPZ740I Bacillus stearothermophilus PZ740 5' CTTAAG 3' GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' BstRZ246I Bacillus stearothermophilus RZ246 5' ATTTAAAT 3' TAAATTTA 5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5' BstSI Bacillus stearothermophilus S183 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, NspIII, OfoI, PunAIBstSCI Bacillus stearothermophilus SC 5' CCNGG 3' GGNCC 5' --- CCNGG--- 3' 3' ---GGNCC --- 5' BstSFI Bacillus stearothermophilus SF 5' CTRYAG 3' GAYRTC 5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' BstSNI Bacillus stearothermophilus SN 5' TACGTA 3' ATGCAT 5' ---TAC GTA--- 3' 3' ---ATG CAT--- 5' BstSWI Bacillus stearothermophilus SW 5' ATTTAAAT 3' TAAATTTA 5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5' BstT7I Bacillus stearothermophilus T7 5' TGATCA 3' ACTAGT 5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' AbaI, BclI, BsiQI, BspXII, FbaI, Ksp22I, ParIBstT9I Bacillus stearothermophilus T9 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AspAI, Bse64I, BstT10I, BstPI, Eco91I, EcoO128I, NspSAII, PspEIBstT10I Bacillus stearothermophilus T10 5' GGTNACC 3' CCANTGG 5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' AspAI, BseT10I, BstEII, BstT10I, Eco91I, EcoO128I, NspSAII, PspEIBst31TI Bacillus stearothermophilus 31T 5' GGATC 3' CCTAG 5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' BstTS5I Bacillus stearothermophilus TS5 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BstUI Bacillus stearothermophilus U458 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, BceBI, BepI, Bpu95I, BtkI, Csp68KVI, FauBII, MvnI, SelIBst2UI Bacillus stearothermophilus 2U 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, SniI, SslI, TaqXI, ZanIBstVI Bacillus stearothermophilus V 5' CTCGAG 3' GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BluI, BssHI, MlaAI, PanI, SauLPII, SciI, SlaI, StrI, XhoIBstV1I Bacillus stearothermophilus V1 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71IBstV2I Bacillus stearothermophilus V2 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BstXI Bacillus stearothermophilus X1 5' CCAN6TGG 3' GGTN6ACC 5' ---CCANNNNN NTGG--- 3' 3' ---GGTN NNNNNACC--- 5' BstX2I Bacillus stearothermophilus X2 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' BstYI 1SDO Bacillus stearothermophilus Y406 5' RGATCY 3' YCTAGR 5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' BstZI Bacillus stearothermophilus Z130 5' CGGCCG 3' GCCGGC 5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' AaaI, BseX3I, EagI, EclXI, Eco52I, SenPT16I, XmaIIIBstZ17I Bacillus stearothermophilus 38M 5' GTATAC 3' CATATG 5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5'
Bsu - Bv