This article contains a list of the most studied restriction enzymes whose names start with Ba to Bc inclusive. It contains approximately 120 enzymes.
The following information is given:
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Restriction enzymes
Ba - Bc
Enzyme PDB code Source Recognition sequence Cut IsoschizomersBac36I Bacillus alcalophilus 36 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIVBaeI Bacillus sphaericus 5' ACN4GTAYC 3' TGN4CATYG 5' ---ACN4GTAYCN6NNNNNN --- 3' 3' ---TGN4CATYGN6N NNNNN--- 5' — None in May 2010 —BalI Brevibacterium albidum 5' TGGCCA 3' ACCGGT 5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5' Bal228I Bacillus alcalophilus 228 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, AvcI, BavAII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIVBamHI 1BAM Bacillus amyloliquefaciens H 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, Bce751I, Bsp98I, Bsp4009I, BspAAIII, CelI, Nsp29132II, NspSAIV, SolI, SurIBamNII Bacillus amyloliquefaciens N 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BcuAI, BsrAI, CauI, EagMI, FdiI, HgiCII, HgiJI, SinIBanI Bacillus aneurinolyticus 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' AccB1I, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAIBanII Bacillus aneurinolyticus 5' GRGCYC 3' CYCGRG 5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5' BanIII Bacillus aneurinolyticus 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaIBanAI Bacillus anthracis 5' GGCC 3' CCGG 5' ---GG CC--- 3' 3' ---CC GG--- 5' BasI Bacillus sp. 5' CCAN5TGG 3' GGTN5ACC 5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, Esp1396I, PflBI, PflMI, Van91IBauI Bacillus aquaemaris RFL1 5' CACGAG 3' GTGCTC 5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5' BavI Bacillus alvei 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' BavAI Bacillus alvei A 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' BavAII Bacillus alvei A 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, AvcI, BavBII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIVBavBI Bacillus alvei B 5' CAGCTG 3' GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5' BavBII Bacillus alvei B 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, BshKI, BspBII, Bsu54I, FmuI, Pde12IBavCI Bacillus alvei C 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' AagI, BanIII, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaIBbeI Bifidobacterium breve YIT4006 5' GGCGCC 3' CCGCGG 5' ---GGCGC C--- 3' 3' ---C CGCGG--- 5' BbiII Bifidobacterium bifidum YIT4007 5' GRCGYC 3' CYGCRG 5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' AcyI, AhaII, BbiII, HgiHII, Hin1I, Hsp92I, Msp17I, PamIIBbi24I Bifidobacterium bifidum S-24 5' ACGCGT 3' TGCGCA 5' ---A CGCGT--- 3' 3' ---TGCGC A--- 5' BbrI Bordetella bronchiseptica 4994 5' AAGCTT 3' TTCGAA 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' Bbr7I Bacillus brevis 7 5' GAAGAC 3' CTTCTG 5' ---GAAGACN6N NNNNN--- 3' 3' ---CTTCTGN6NNNNNN --- 5' BbrPI Bacillus brevis 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BcoAI, Eco72I, PmaCI, PmlI, PspCIBbsI Bacillus laterosporus 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BbuI Bacillus circulans 5' GCATGC 3' CGTACG 5' ---GCATG C--- 3' 3' ---C GTACG--- 5' BbvI Bacillus brevis 5' GCAGC 3' CGTCG 5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' AlwXI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1IBbvII Bacillus brevis 80 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' Bbv12I Bacillus brevis 12 5' GWGCWC 3' CWCGWG 5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5' Alw21I, AspHI, Bsh45I, BsiHKAI, HgiAI, HpyF46II, MspV281IBbv16II Bacillus brevis 16 5' GAAGAC 3' CTTCTG 5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' BbvAI Bacillus brevis A 5' GAAN4TTC 3' CTTN4AAG 5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5' Asp700I, MroXI, PdmI, XmnIBbvAII Bacillus brevis A 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BavCI, Bci29I, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21IBbvAIII Bacillus brevis A 5' TCCGGA 3' AGGCCT 5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5' AccIII, Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Bsu23I, Kpn2IBbvBI Bacillus brevis B 5' GGYRCC 3' CCRYGG 5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5' BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAIBbvCI Bacillus brevis 5' CCTCAGC 3' GGAGTCG 5' ---CC TCAGC--- 3' 3' ---GGAGT CG--- 5' AbeIBca77I Bacillus caldolyticus 5' WCCGGW 3' WGGCCW 5' ---W CCGGW--- 3' 3' ---WGGCC W--- 5' BccI Bacteroides caccae 5' CCATC 3' GGTAG 5' ---CCATCNNNN N--- 3' 3' ---GGTAGNNNNN --- 5' Bce4I Bacillus cereus B4 5' GCN7GC 3' CGN7CG 5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' Bce22I Bacillus cereus 22 5' GGNCC 3' CCNGG 5' ---G GNCC--- 3' 3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, FmuI, Pde12IBce83I Bacillus cereus 83 5' CTTGAG 3' GAACTC 5' ---CTTGAGN13NNN --- 3' 3' ---GAACTCN13N NN--- 5' Bce243I Bacillus cereus 5' GATC 3' CTAG 5' --- GATC--- 3' 3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368IBce751I Bacillus cereus 751 5' GGATCC 3' CCTAGG 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' BamHI, Bce751I, BnaI, Bsp98I, Bsp4009I, BstI, NspSAIV, Pfl8IBceAI Bacillus cereus 1315 5' ACGGC 3' TGCCG 5' ---ACGGCN11N NN--- 3' 3' ---TGCCGN11NNN --- 5' BceBI Bacillus cereus 1323 5' CGCG 3' GCGC 5' ---CG CG--- 3' 3' ---GC GC--- 5' AccII, Bsh1236I, BtkI, Csp68KVI, FalII, FauBII, FnuDII, SelI, ThaIBceCI Bacillus cereus 1195 5' GCN7GC 3' CGN7CG 5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' BcefI Bacillus cereus fluorescens 5' ACGGC 3' TGCCG 5' ---ACGGCN10NN N--- 3' 3' ---TGCCGN10NNN --- 5' BcgI Bacillus coagulans 5' CGAN6TGC 3' GCTN6ACG 5' ---CGAN6TGCN9NNN --- 3' 3' ---GCTN6ACGN9N NN--- 5' Bci29I Bacillus circulans 29 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BavCI, BciBI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21IBciBI Bacillus circulans B 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BavCI, BcmI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21IBciBII Bacillus circulans B 5' CCWGG 3' GGWCC 5' ---CC WGG--- 3' 3' ---GGW CC--- 5' AjnI, ApyI, BptI, Bst1I, BstOI, BstM6I, Bst2UI, EcoRII, MvaIBciVI Bacillus circulans 5' GTATCC 3' CATAGG 5' ---GTATCCN4NN --- 3' 3' ---CATAGGN4N N--- 5' BfuIBclI Bacillus caldolyticus 5' TGATCA 3' ACTAGT 5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' AbaI, BsiQI, BspXII, BstT7I, FbaI, Ksp22I, ParIBcmI Bacillus sp. 5' ATCGAT 3' TAGCTA 5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' BsuTUI, Bsu15I, BavCI, Bli86I, BspZEI, Rme21I, BseCI, BdiIBcnI 2ODH Bacillus centrosporus RFL1 5' CCSGG 3' GGSCC 5' ---CC SGG--- 3' 3' ---GGS CC--- 5' AhaI, AseII, AsuC2I, BpuMI, CauII, Eco1831I, HgiS22I,Kpn49kII, NciIBcoI Bacillus coagulans SM 1 5' CYCGRG 3' GRGCYC 5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' Ama87I, AquI, BsoBI, BstSI, Eco88I, NspSAI, OfoI, PunAIBco5I Bacillus coagulans 5 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' Bco27I Bacillus coagulans 27 5' CCGG 3' GGCC 5' ---C CGG--- 3' 3' ---GGC C--- 5' Bco116I Bacillus coagulans 116 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' Bco118I Bacillus coagulans 118 5' RCCGGY 3' YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' BcoAI Bacillus coagulans AUCM B-732 5' CACGTG 3' GTGCAC 5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5' AcvI, BbrPI, Eco72I, PmaCI, PmlI, PspCIBcoKI Bacillus coagulans 5' CTCTTC 3' GAGAAG 5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' BcuI Bacillus coagulans Vs 29-022 5' ACTAGT 3' TGATCA 5' ---A CTAGT--- 3' 3' ---TGATC A--- 5' AhII, AclNI, SpeIBcuAI Bacillus cereus BKM B-814 5' GGWCC 3' CCWGG 5' ---G GWCC--- 3' 3' ---CCWG G--- 5' BamNxI, BsrAI, Csp68KI, EagMI, FssI, HgiCII, HgiJI, SinI
Notes