Guidoni is an Italian surname. Notable people with the surname include:
Alessandro Guidoni (1880–1928), Italian Air Force general Dorival Guidoni Junior (born 1972), Brazilian retired footballer Jean Guidoni (1952–2025), French singer and songwriter Stefano Guidoni (born 1971), Italian footballer Umberto Guidoni (born 1954), Italian astrophysicist, science writer and a former ESA astronaut,
See also 10605 Guidoni, asteroid Guideschi Valter Giuliani