Gillespie House may refer to:
in the United States (by state then city) Gillespie House (Sarasota, Florida), listed on the NRHP in Sarasota County Gillespie-Jackson House, Starkville, Mississippi, listed on the NRHP in Oktibbeha County Gillespie House (Guilderland, New York), listed on the NRHP in Albany County Col. George Gillespie House, Limestone, Tennessee, listed on the NRHP in Washington County James Gillespie House, Louisville, Tennessee, listed on the NRHP in Blount County