Burkholder is a surname. Notable people with the surname include:
Dave Burkholder (contemporary), Canadian college ice hockey coach Donald Burkholder (1927–2013), American mathematician J. Peter Burkholder (born 1954), American musicologist James B. Burkholder (1918–2006), American army officer, activist for peace and social justice issues JoAnn Marie Burkholder (born 1953), American ecologist Mabel Burkholder (1881–1973), Canadian historian and author Max Burkholder (born 1997), American actor Seth Burkholder (born 1982), American football player Steve Burkholder (contemporary), Republican politician from Colorado, mayor of Lakewood 1999–2007 Walter H. Burkholder (1898–1977), American plant pathologist