Line 16 may refer to:
China Line 16 (Beijing Subway) Line 16 (Guangzhou Metro) Line 16 (Hangzhou Metro) Line 16 (Shanghai Metro) Line 16 (Shenzhen Metro) Line 16 (Wuhan Metro) Line 16 (Xi'an Metro)
Other countries Paris Metro Line 16, a line under construction in France Line 16 (São Paulo Metro), in Brazil
See also List of public transport routes numbered 16