The Dutch Tweede Divisie in the 1965–66 season was contested by 30 teams, divided in two groups. It would be the last season the Tweede Divisie would be divided in two. From next season onwards, all teams would participate in one league. This also meant that more teams than before were promoted to the Eerste Divisie. No teams had to relegate to amateur football.
New entrants and group changes
Group A Relegated from the Eerste Divisie: Veendam Entered from the B-group: HVC SC Gooiland (last season playing as 't Gooi)
Group B Relegated from the Eerste Divisie: Excelsior Entered from the A-group: RCH HFC Haarlem HFC EDO FC Den Bosch played as BVV last season
Group A
Group B
See also 1965–66 Eredivisie 1965–66 Eerste Divisie
References Netherlands - List of final tables (RSSSF)