Gstrein is a surname. Notable people with the surname include:
Bernhard Gstrein (born 1965), Austrian alpine skier Fabio Gstrein (born 1997), Austrian alpine skier Hugo Gstrein, Austrian cross-country skier Josl Gstrein (1917–1980), Austrian cross-country and Nordic combined skier Norbert Gstrein (born 1961), Austrian writer