Gherardini is an Italian surname. Notable people with the surname include:
Gherardini family of Montagliari, aristocratic family of Florence Alessandro Gherardini (1655–1723), Italian painter of Baroque Florence Jacopo Schettini Gherardini (born 1965), Italian economist Maurizio Gherardini (born 1955), Italian sportsman, general manager for the Fenerbahçe Basketball Lisa Gherardini (1479–1542), woman depicted in the Mona Lisa painting Melchiorre Gherardini (1607–1668), Italian painter, known as Ceranino Stefano Gherardini (1695–1755), Italian genre painter Tommaso Gherardini (1715–1797), Italian painter of late 18th-century Florence
See also Gherardi Gherardesca Girardin, surname taken by members of the Gherardini family in France